NUCLEIC-ACID JUNCTIONS AND LATTICES
NUCLEIC-ACID JUNCTIONS AND LATTICES
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DOI：
10.1016/0022-5193(82)90002-9
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发表时间：
1982-01-01
影响因子：
2
通讯作者：
SEEMAN, NC
中科院分区：
文献类型：
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作者：
SEEMAN, NC
有可能产生寡聚核酸的序列，其将优先缔合以形成迁移不动的连接，而不是线性双链体，如它们通常所做的那样。这些结构是基于沃森-克里克碱基配对的最大化和缺乏序列对称性，而序列对称性通常在生命系统中的类似物中发现。提出了寡核苷酸序列必须满足的标准，以产生这些连接结构。接下来是可生成的连接点，从那里可以产生3-8个双螺旋。每个连接点可以被视为一个大分子价簇，并且各个簇可以直接连接在一起，或者用散布在它们之间的线性DNA片段连接。这种共价连接可以通过使用目前在基因工程研究中使用的粘性末端连接技术以极大的特异性来完成。似乎有可能产生共价连接的三维网络的核酸，这是周期性的连接，也许在空间。
It is possible to generate sequences of oligomeric nucleic acids which will preferentially associate to form migrationally immobile junctions, rather than linear duplexes, as they usually do. These structures are predicated on the maximization of Watson-Crick base pairing and the lack of sequence symmetry customarily found in their analogs in living systems. Criteria are presented which oligonucleotide sequences must fulfill in order to yield these junction structures. The generable junctions are next, from which 3-8 double helices may emanate. Each junction may be treated as a macromolecular valence cluster, and the individual clusters may be linked together directly, or with pieces of linear DNA interspersed between them. This covalent linkage can be done with enormous specificity, using the sticky-ended ligation techniques currently employed in genetic engineering studies. It appears to be possible to generate covalently joined 3-dimensional networks of nucleic acids which are periodic in connectivity and perhaps in space.