A novel potassium channel encoded by Ectocarpus siliculosus virus
A novel potassium channel encoded by Ectocarpus siliculosus virus
复制标题
DOI：
10.1016/j.bbrc.2004.11.125
复制
发表时间：
2005-01-28
影响因子：
3.1
通讯作者：
Gopalakrishnan, M
中科院分区：
文献类型：
--
作者：
Chen, J;Cassar, SC;Gopalakrishnan, M
Kcv是第一个由绿色海藻草履虫囊藻病毒（Parameciumbursariaperellavirus，PBCV-1）编码的病毒性钾通道，在异源系统中表达时，可传导钾离子选择性电流。这种K+通道被认为对PBCV-1的感染和复制很重要。在本研究中，我们确定了一种新的K+通道Kesv，其功能特征在于，编码的Ectocarpus siliculosus病毒感染丝状海洋褐藻。Kesv基因编码124个氨基酸，与Kcv基因的同源性分别为21.8%和37.1%。膜拓扑学程序预测Kesv由三个跨膜结构域组成。当在非洲爪蟾卵母细胞中表达时，Kesv诱导的瞬时K+选择性电流对Ba 2+和金刚烷胺的阻断敏感。因此，Kesv沿着Kcv，构成了一个新兴的病毒钾通道家族，可能在病毒的生命周期中发挥重要作用。(C)2004年爱思唯尔公司All rights reserved.
Kcv, the first identified viral potassium channel encoded by the green algae Paramecium bursaria chlorella virus (PBCV-1), conducted K+ selective currents when expressed in heterologous systems. This K+ channel was proposed to be important for PBCV-1 infection and replication. In the present study, we identified and functionally characterized a novel K+ channel Kesv, encoded by Ectocarpus siliculosus virus that infects filamentous marine brown algae. Kesv encodes a protein of 124 amino acids and is 21.8% identical and 37.1% homologous to Kcv. Membrane topology programs predicted that Kesv consists of three transmembrane domains. When expressed in Xenopus oocytes, Kesv induced largely instantaneous, K+ selective currents that were sensitive to block by Ba2+ and amantadine. Thus, Kesv along with Kcv, constitutes an emerging family of viral potassium channels, which may play important roles in the life cycle of viruses. (C) 2004 Elsevier Inc. All rights reserved.