Introgression of a quantitative trait locus for yield from Glycine soja into commercial soybean cultivars
Introgression of a quantitative trait locus for yield from Glycine soja into commercial soybean cultivars
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DOI：
10.1007/s00122-002-1071-5
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发表时间：
2003-02-01
影响因子：
5.4
通讯作者：
Delannay, X
中科院分区：
文献类型：
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作者：
Concibido, VC;La Vallee, B;Delannay, X
外来种质在拓宽大多数作物遗传基础方面的价值已被多次证明。然而，使用外来种质所涉及的困难限制了它们在植物育种中的实用性。不需要的联系常常阻碍有益的外来基因成功整合到商业品系中。因此，在传统育种中使用外来品种使得作物改良过程成为一项乏味、耗时且昂贵的工作。分子标记的可用性使得分离特定基因组区域并将其转移到具有最小连锁阻力的商业品种成为可能。我们通过评估 HS-1 和 PI 407305 杂交的 265 个 BC2 个体群体，发现了大豆 (Siebold 和 Zucc.) 的增产数量性状位点 (QTL)。产量 QTL 位于大豆 [Glycine max (L.) Merrill] 遗传连锁图谱的连锁群 B2(U26) 上。在一项为期 2 年的多地点研究中，QTL 位点携带 PI 407305 单倍型的个体比不包含外来单倍型的个体表现出 9.4% 的产量优势。当在两个位置的更均匀的“HS-1 样”背景中进行测试时，我们观察到携带 PI 407305 单倍型的品系具有 8% 的产量优势。我们进一步评估了各种优良大豆遗传背景中的QTL效应。产量效应仅在六种遗传背景中的两种中一致观察到。在QTL位点携带PI 407305单倍型的个体在跨地点的产量试验中具有9%的产量优势。尽管这种产量QTL在遗传背景下的适应性有限，但本研究证明了外来种质在大豆增产方面的潜力。
The value of exotic germplasm in broadening the genetic base of most crops has been demonstrated many times. However, the difficulties involved in working with exotic germplasm have limited their utility in plant breeding. Unwanted linkages often thwart the successful incorporation of beneficial exotic genes into commercial lines. Thus, the use of exotics in traditional breeding makes the process of crop improvement a tedious, time-consuming and expensive endeavor. The availability of molecular markers makes it possible to isolate specific genomic regions and transfer them into commercial varieties with minimal linkage drag. We found a yield-enhancing quantitative trait locus (QTL) from Glycine soja (Siebold and Zucc.) by evaluating a population of 265 BC2 individuals from a cross between HS-1 and PI 407305. The yield QTL was located on linkage group B2(U26) of the soybean [Glycine max (L.) Merrill] genetic linkage map. In a 2-year, multi-location study, individuals carrying the PI 407305 haplotype at the QTL locus demonstrated a 9.4% yield advantage over individuals that did not contain the exotic haplotype. When tested in a more uniform "HS-1-like" background in two locations, we observed an 8% yield advantage for lines that carry the PI 407305 haplotype. We further assessed the QTL effect in various elite soybean genetic backgrounds. The yield effect was consistently observed in only two of six genetic backgrounds. Individuals carrying the PI 407305 haplotype at the QTL locus had a 9% yield advantage in yield trials across locations. Despite the limited adaptability of this yield-QTL across genetic backgrounds, this study demonstrates the potential of exotic germplasm for yield enhancement in soybean.