Analysis of n-6 / n-3 ratio of polyunsaturated fatty acids in human serum by reactive thermal desorption-gas chromatography using a vertical microfurnace pyrolyzer
Analysis of n-6 / n-3 ratio of polyunsaturated fatty acids in human serum by reactive thermal desorption-gas chromatography using a vertical microfurnace pyrolyzer
复制标题
使用立式微型炉热解器通过反应热解吸气相色谱法分析人血清中多不饱和脂肪酸的 n-6 / n-3 比率
DOI：
10.1016/j.jaap.2022.105758
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发表时间：
2022
影响因子：
6
通讯作者：
Watanabe Atsushi
中科院分区：
文献类型：
--
作者：
Ishida Yasuyuki;Furuta Shiori;Aiba Amane;Kuzumaki Akane;Miyazawa Daisuke;Watanabe Atsushi
采用热辅助水解-甲基化-气相色谱法（THM-GC），采用立式微炉裂解器和火焰离子化检测器（FID），快速分析人血清中多不饱和脂肪酸（PUFA）的化学组成。在这里，作者使用了反应性热解吸（RTD）方法，其中2 μL血清样品在250 °C下进行THM反应20 s，以高选择性和灵敏度检测PUFA组分。所得色谱图清楚地显示了一系列脂肪酸甲酯，包括PUFA，没有任何来自血清样品基质组分的干扰峰。此外，所观察到的化学组成和n-6 / n-3比例的PUFA组分是在良好的协议与通过传统的分析技术，包括溶剂萃取和甲基转移。
Thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) using a vertical microfurnace pyrolyzer and a flame ionization detector (FID) was applied to rapid analysis of chemical composition for polyunsaturated fatty acid (PUFA) components in human serum. Here, the authors used a reactive thermal desorption (RTD) method, where 2 μL of a serum sample was subjected to the THM reaction at 250 °C for 20 s, to detect PUFA components with high selectivity and sensitivity. The resulting chromatograms clearly showed a series of fatty acid methyl esters including PUFAs without any interference peaks derived from the matrix components of the serum samples. Furthermore, the observed chemical composition and n-6 / n-3 ratio for PUFA components were in good agreement with those obtained by a conventional analytical technique involving solvent extraction and transmethylation.