Differential expression of Epstein-Barr virus-encoded RNA and several tumor-related genes in various types of nasopharyngeal epithelial lesions and nasopharyngeal carcinoma using tissue microarray analysis
Differential expression of Epstein-Barr virus-encoded RNA and several tumor-related genes in various types of nasopharyngeal epithelial lesions and nasopharyngeal carcinoma using tissue microarray analysis
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DOI：
10.1016/j.humpath.2006.01.010
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发表时间：
2006-05-01
期刊：
影响因子：
3.3
通讯作者：
Li, GY
中科院分区：
文献类型：
--
作者：
Fan, SQ;Ma, J;Li, GY
研究表明，EB病毒（Epstein-Barr virus，EBV）感染、遗传变异和环境因素在鼻咽癌（nasopharyngeal carcinoma，NPC）的发生发展中起重要作用，但其确切机制尚不清楚。本研究的目的是利用组织芯片分析探讨EB病毒编码的小RNA-1（EBER-1）和几个肿瘤相关基因的差异表达是否与NPC癌变相关。对148例鼻咽癌和164例不同形态的非癌鼻咽上皮（NPE）的组织芯片进行免疫组化和原位杂交。结果发现，鼻咽癌组织中EBER-1杂交信号、p53、p21 ras和bcl-2蛋白的过度表达以及p16和p27蛋白的缺失表达均显著高于正常和增生的NPE（P
Studies have revealed that Epstein-Barr virus (EBV) infection, genetic aberration, and environmental factors are of importance in the development of nasopharyngeal carcinoma (NPC), although the definite mechanism remains to be fully elucidated. The aim of our study is to investigate using tissue microarray analysis whether differential expression of EBV-encoded small RNA-I (EBER-1) and several tumor-related genes were associated with NPC carcinogenesis. Immunohistochemistry and in situ hybridization were performed on tissue microarrays containing 148 NPCs and 164 noncancerous nasopharyngeal epithelia (NPE) with different morphologic features. We found that overexpressions of EBER-1 hybridization signals, p53, p21ras, and bcl-2 proteins and loss expressions of p 16 and p27 proteins were significantly increased in NPC tissues compared with normal NPE and hyperplastic NPE (P