Antioxidant efficacy of (-)-loliolide isolated fromSargassum horneriagainst AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
Antioxidant efficacy of (-)-loliolide isolated fromSargassum horneriagainst AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
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DOI：
10.1007/s10811-020-02154-9
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发表时间：
2020-06-25
影响因子：
3.3
通讯作者：
Lee, Dae-Sung
中科院分区：
文献类型：
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作者：
Kim, Hyun-Soo;Wang, Lei;Lee, Dae-Sung
已知海藻衍生化合物具有多种生物活性。本研究评价了从角状马尾藻（Sargassum horneriin）中分离的单萜类化合物（-）-loliperin在Vero细胞和斑马鱼模型中的抗氧化能力。（-）-Lolipidine从anS的氯仿部分纯化。使用高效离心分配色谱法（HPCPC）对霍纳甲醇提取物进行纯化。发现HPCPC是分离（-）-棒棒糖的有效方法，产生高纯度产物。电子自旋共振评价表明（-）-萝芙木素具有显著的烷基自由基清除活性（IC 50：0.043 +/- 0.005 mg mL（-1））。此外，我们的研究表明，（-）-萝芙木素对Vero细胞活力具有剂量依赖性的保护作用，对抗2，2 '-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）诱导的细胞内活性氧（ROS）。此外，（-）-loliprocyte减少脂质过氧化反应在AAPH诱导的斑马鱼胚胎，同时改善存活率和细胞死亡的剂量依赖性的方式。这些结果揭示了（-）-棒棒糖的抗氧化潜力。未来的研究强调了S.角蛋白是制造功能性食品所必需的成分。
Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (-)-loliolide, a monoterpenoid isolated fromSargassum horneriin Vero cells and zebrafish models. (-)-Loliolide was purified from the chloroform fraction of anS. hornerimethanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (-)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (-)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 +/- 0.005 mg mL(-1)). In addition, our study showed that (-)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2 '-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (-)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (-)-loliolide. Future studies highlighting the potential applications ofS. horneriin manufacturing functional food ingredients are required.