Anticancer Activities of an Anthocyanin-Rich Extract From Black Rice Against Breast Cancer Cells In Vitro and In Vivo
Anticancer Activities of an Anthocyanin-Rich Extract From Black Rice Against Breast Cancer Cells In Vitro and In Vivo
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DOI：
10.1080/01635581.2010.494821
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Ling Wenhua
中科院分区：
文献类型：
--
作者：
Chang Hui;Yu Bin;Ling Wenhua
花色苷广泛存在于人类饮食中，具有多种保健作用。本研究探讨富含花青素的黑米提取物（AEBR）对乳腺癌细胞的体外和体内抗癌作用。AEBR降低了乳腺癌细胞系MCF-7（ER+，HER 2/neu-）、MDA-MB-231（ER-，HER 2/neu-）和MDA-MB-453（ER-，HER 2/neu+）的活力，并通过激活半胱天冬酶级联、切割聚（ADP-核糖）聚合酶（PARP）、去极化线粒体膜电位和释放细胞色素C，在体外通过内源性途径诱导MDA-MB-453细胞凋亡。对携带MDA-MB-453细胞异种移植物的BALB/c裸鼠经口给予AEBR（100 mg/kg/天），通过抑制肿瘤组织中血管生成因子MMP-9、MMP-2和uPA的表达，显著抑制肿瘤生长和血管生成。总之，这项研究表明，AEBR对人乳腺癌细胞在体外和体内的抗癌作用，诱导凋亡和抑制血管生成。
Anthocyanins widely present in human diet and have a variety of health effects. This study investigates the anticancer effects of an anthocyanin-rich extract from black rice (AEBR) on breast cancer cells in vitro and in vivo. AEBR reduced the viability of breast cancer cell lines MCF-7 (ER+, HER2/neu-), MDA-MB-231 (ER-, HER2/neu-), and MDA-MB-453 (ER-, HER2/neu+) and induced apoptosis in MDA-MB-453 cells via the intrinsic pathway in vitro by activating caspase cascade, cleaving poly (ADP-ribose) polymerase (PARP), depolarizing mitochondrial membrane potential, and releasing cytochrome C. Oral administration of AEBR (100 mg/kg/day) to BALB/c nude mice bearing MDA-MB-453 cell xenografts significantly suppressed tumor growth and angiogenesis by suppressing the expression of angiogenesis factors MMP-9, MMP-2, and uPA in tumor tissue. Altogether, this study suggests the anticancer effects of AEBR against human breast cancer cells in vitro and in vivo by inducing apoptosis and suppressing angiogenesis.