Characterization of mucins in human middle ear and Eustachian tube
Characterization of mucins in human middle ear and Eustachian tube
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DOI：
10.1152/ajplung.2001.280.6.l1157
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发表时间：
2001-06-01
影响因子：
4.9
通讯作者：
Ho, SB
中科院分区：
文献类型：
--
作者：
Lin, JZ;Tsuprun, V;Ho, SB
粘蛋白是中耳和咽鼓管粘液纤毛运输系统中重要的糖蛋白。在生理和病理条件下，粘蛋白在该系统中的表达知之甚少。本研究证实了MUC5B、MUC5AC、MUC4和MUC1在人咽鼓管中的表达，而在非炎症中耳中仅有MUC5B粘蛋白的表达。在慢性中耳炎(COM)和粘液性中耳炎(MOM)患者中，MUC5B和MUC4粘蛋白基因分别上调4.2倍和6倍。这种粘蛋白基因的上调伴随着中耳粘膜中MUC5B和MUC4产生细胞的增加。COM和MOM分泌物的电子显微镜显示存在链状聚合粘蛋白。这些结果表明，中耳和咽鼓管上皮细胞表达不同的粘蛋白，而MUC5B和MUC4粘蛋白在病变中耳中大量产生和分泌。这些粘蛋白可能会在中耳腔内形成厚厚的粘液渗出，损害中耳的功能。
Mucins are important glycoproteins in the mucociliary transport system of the middle ear and Eustachian tube. Little is known about mucin expression within this system under physiological and pathological conditions. This study demonstrated the expression of MUC5B, MUC5AC, MUC4, and MUC1 in the human Eustachian tube, whereas only MUC5B mucin expression was demonstrated in noninflamed middle ears. MUC5B and MUC4 mucin genes were upregulated 4.2- and 6-fold, respectively, in middle ears with chronic otitis media (COM) or mucoid otitis media (MOM). This upregulation of mucin genes was accompanied by an increase of MUC5B- and MUC4-producing cells in the middle ear mucosa. Electron microscopy of the secretions from COM and MOM showed the presence of chainlike polymeric mucin. These data indicate that the epithelium of the middle ear and Eustachian tube expresses distinct mucin profiles and that MUC5B and MUC4 mucins are highly produced and secreted in the diseased middle ear. These mucins may form thick mucous effusion in the middle ear cavity and compromise the function of the middle ear.