Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in murine gastrointestinal musculature
Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in murine gastrointestinal musculature
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DOI：
10.1679/aohc.72.107
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发表时间：
2009-09-01
影响因子：
--
通讯作者：
Nojyo, Yoshiaki
中科院分区：
文献类型：
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作者：
Iino, Satoshi;Nojyo, Yoshiaki
Cajal间质细胞（interstitial cells of Cajal，ICC）分布于胃肠道的肌肉组织中，对胃肠运动起着重要的调节作用.另一种称为成纤维细胞样细胞（FLC）的间充质细胞也被报道与ICC并列。在这项研究中，我们研究了FLC在小鼠胃肠道肌肉组织的免疫组化特性，使用抗体小电导钙激活的K+通道3（SK 3），血小板衍生生长因子受体α（PDGFR α），和CD 34。在整个胃肠道的肌肉组织中观察到SK 3免疫阳性（SK 3-ip）细胞。这些SK 3-ip细胞与通过c-Kit免疫反应性鉴定的ICC不同。在肌层中，SK 3-ip细胞呈双极型，与肌内ICC和神经纤维束相连。在肌间神经节中，多极形状的SK 3-ip细胞包围肌间神经节。位于亚浆膜平面的SK 3-ip细胞以其分支突起形成细胞网络。SK 3-ip细胞在ICC缺陷型W-v/W-v突变小鼠中的分布模式与正常小鼠相似。我们还证明，SK 3-ip细胞显示出强烈的PDGFR α免疫反应性，这是以前在FLC中检查。然而，在SK 3-ip细胞中未观察到人FLC的标志物之一的CD 34免疫反应性，除了皮下FLC。因此，我们的观察结果表明，SK 3-和PDGFR α-双免疫阳性细胞是FLC在小鼠胃肠道肌肉组织和行为作为一个基本的细胞元素在整个胃肠道肌肉组织。
In the gastrointestinal musculature, interstitial cells of Cajal (ICC) distribute and regulate the gastrointestinal motility. Another type of mesenchymal cell, known as the fibroblast-like cell (FLC), has also been reported to be juxtaposed to the ICC. In this study, we examined the immunohistochemical properties of FLC in the murine gastrointestinal musculature using antibodies to small conductance Ca2+-activated K+ channel 3 (SK3), platelet-derived growth factor receptor alpha (PDGFR alpha), and CD34. SK3-immunopositive (SK3-ip) cells were observed in the musculature throughout the gastrointestinal tract. These SK3-ip cells were distinct from the ICC that were identified by c-Kit immunoreactivity. In the muscular layers, SK3-ip cells were bipolar in shape and were associated with the intramuscular ICC and nerve fiber bundles. In the myenteric layer multipolar-shaped SK3-ip cells encompassed the myenteric ganglia. SK3-ip cells in the subserosal plane formed a cellular network with their ramified processes. The distribution pattern of the SK3-ip cells in the ICC-deficient W-v/W-v mutant mice was similar to that in normal mice. We also demonstrated that SK3-ip cells showed the intense PDGFR alpha immunoreactivity that was previously examined in FLC. However, CD34 immunoreactivity, one of the markers of human FLC, was not observed in SK3-ip cells with the exception of subserosal FLC. Thus, our observations indicate that SK3- and PDGFR alpha-double immunopositive cells are FLC in the murine gastrointestinal musculature and behave as a basic cellular element throughout the gastrointestinal musculature.