Phylogenetics and comparative analysis of the mitochondrial genomes of three violet‐ringed octopuses
Phylogenetics and comparative analysis of the mitochondrial genomes of three violet‐ringed octopuses
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DOI：
10.1111/zsc.12359
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发表时间：
2019-07
影响因子：
2.5
通讯作者：
Yanli Tang;Xiaodong Zheng;H. Zhong;Qi Li
中科院分区：
文献类型：
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作者：
Yanli Tang;Xiaodong Zheng;H. Zhong;Qi Li
两栖章鱼雷克斯A. neglectus和A.参见ovulum导致它们的系统发育地位不明，关系不清。本研究对中国沃茨中这三种鱼类的线粒体全基因组进行了测序，并进行了比较，以阐明它们之间的亲缘关系。线粒体基因组的长度从15，646 bp到15，814 bp不等，蛋白质编码基因的A + T含量和GC偏斜几乎没有变化。与此相反，基于密码子使用的树状图和三种章鱼的基因排列都显示A。雷克斯与A.而忽略A.参见排卵首次利用5个数据集和2种方法（最大似然法和贝叶斯推断法）对这3种蝗虫的系统发育关系进行了研究。结果表明，结合蛋白质编码基因和RNA基因（PR）的数据集是最佳的分析43头足类之间的关系。所有的系统发育树将头足类划分为10个类群，并支持始羊齿目、小羊齿目、乌贼科和乌贼科的单系性。在这项研究中，异乌贼科被归类为乌贼科的姐妹。使用所有数据集构建的树强有力地支持单系的属Amphioctopus。值得注意的是，A.雷克斯与A.而忽略A.参见卵，虽然这三个物种共享的特点是紫色环的黑暗ocelli。
Similar morphological characters and little molecular data of Amphioctopus rex, A. neglectus and A. cf. ovulum resulted in their unknown phylogenetic statuses and equivocal relationships. In this study, the complete mitochondrial genomes of these three species collected in Chinese waters were sequenced and compared with each other to clarify the relationships among them. The lengths of the mitochondrial genomes varied from 15,646 bp to 15,814 bp, and the A + T content and GC skew for protein‐coding genes showed little variation. In contrast, both a dendrogram based on codon usage and the gene arrangements of the three octopuses showed that A. rex was more closely related to A. neglectus than to A. cf. ovulum. Five data sets and two methods (maximum likelihood and Bayesian inference) were utilized for the first time to explore the phylogenetic relationships among these three species in Octopodidae. The results indicated that a data set combining protein‐coding genes and RNA genes (PR) was optimal for analysing the relationships among 43 cephalopods. All of the phylogenetic trees divided the cephalopods into 10 taxa and supported the monophyly of Oegopsida, Myopsida, Sepiidae and Octopodidae. In this study, Idiosepiidae was classified as sister to Sepiolidae. Trees constructed using all data sets robustly supported the monophyly of the genus Amphioctopus. Notably, A. rex was more closely related to A. neglectus than to A. cf. ovulum, although these three species share the characteristic of violet rings on dark ocelli.