Electrochemical Dissolved Oxygen Sensor-Integrated Platform for Wireless In Situ Bioprocess Monitoring
Electrochemical Dissolved Oxygen Sensor-Integrated Platform for Wireless In Situ Bioprocess Monitoring
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DOI：
10.1016/j.snb.2020.128381
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发表时间：
2020-10-01
影响因子：
8.4
通讯作者：
Ghodssi, Reza
中科院分区：
文献类型：
--
作者：
Stine, Justin M.;Beardslee, Luke A.;Ghodssi, Reza
本工作提出了一种生物处理在线设备（bPod）平台，能够实时原位监测生物反应器细胞培养参数，如溶解氧（DO）。bPod是一个集成系统，由恒电位仪模拟前端（AFE）、蓝牙低功耗（BLE）微控制器和化学传感器组成，这里以Clark型DO传感器为例，可以监测溶解氧含量。Clark型电化学传感器执行DO百分比饱和度的计时电流测量，并且BLE微控制器在浸没在水性介质中时将数据无线传输到智能手机。经过几次电极设计修改后，bPod显示出对应于DO百分比饱和水平的线性电化学电流响应，灵敏度为37.5 nA/DO%，检测限为8.26 DO%，涵盖了生物反应器内哺乳动物培养过程相关的浓度范围。无线bPod提供了一个自由浮动的胶囊架构，用于监测DO，并可适用于一系列电化学传感器，针对不同的生物过程监测应用的不同过程参数。
This work presents a bio-processing online device (bPod) platform, capable of real-time in situ monitoring of bioreactor cell culture parameters, such as dissolved oxygen (DO). The bPod is an integrated system comprised of a potentiostat analog-front-end (AFE), a Bluetooth Low Energy (BLE) microcontroller, and a chemical sensor, exemplified here by a Clark-type DO sensor, which enables monitoring of dissolved oxygen content. The Clark-type electrochemical sensor performs chronoamperometric measurement of DO percent saturation, and the BLE microcontroller wirelessly transmits data to a smartphone while submerged in aqueous media. After several electrode design modifications, the bPod showed a linear electrochemical current response corresponding to DO percent saturation levels with a sensitivity of 37.5 nA/DO% and limit of detection of 8.26 DO%, covering concentration ranges relevant for mammalian culture processes within bioreactors. The wireless bPod provides a free-floating capsule architecture for monitoring DO and can be adapted for an array of electrochemical sensors, targeting different process parameters for diverse bioprocess monitoring applications.