Development and genetic analysis of wheat double substitution lines carrying Hordeum vulgare 2H and Thinopyrum intermedium 2Ai2 chromosomes
Development and genetic analysis of wheat double substitution lines carrying Hordeum vulgare 2H and Thinopyrum intermedium 2Ai2 chromosomes
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大麦2H和中间堇草2Ai2染色体小麦双替代系的发育及遗传分析
DOI：
10.1016/j.cj.2018.11.003
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发表时间：
2019
期刊：
影响因子：
6.6
通讯作者：
Ye Xingguo
中科院分区：
文献类型：
--
作者：
Wang Jing;Liu Chang;Guo Xianrui;Wang Ke;Du Lipu;Lin Zhishan;Ye Xingguo
中间偃麦草和大麦是小麦的近缘种，它们携带着许多对小麦性状改良有潜在价值的基因。本研究通过不同小麦品种间的杂交，建立了小麦双代换系。中间体和小麦-大麦二体异代换系，目的是利用Th.中间体和大麦的遗传行为，并研究了外源染色体及其小麦同源染色体的遗传行为。结果表明，我们获得了两种类型的小麦双代换系2D2Ai#2（2B）2H（2A）和2A2Ai#2（2B）2H（2D），它们是由不同的第2组小麦染色体分别被大麦染色体2H和Th所取代。中间染色体2Ai#2。新材料的特点是使用分子标记，基因组素原位杂交（GISH），荧光原位杂交（FISH）。GISH和FISH检测结果表明，双代换系含有42条染色体，其中小麦染色体38条，大麦染色体1对，Th染色体1对。中间染色体特异DNA标记分析表明，新品系中的两对小麦同源群2染色体被一对2H和一对2Ai#2染色体所取代。2H染色体的传递率比2Ai#2高，两条染色体都优先通过雌配子传递。植物学和农艺性状评价表明，与亲本相比，新品系的生长习性和株型相似，但株高、开花期和自交结实性存在差异。用不同探针进行的细胞学观察表明，双代换系在减数分裂前和减数分裂期间表现出几乎正常的遗传行为。新的代换系可用于小麦减数分裂研究和育种计划。
Thinopyrum intermediumand barley are two close relatives of wheat and carry many genes that are potentially valuable for the improvement of various wheat traits. In this study we created wheat double substitution lines by hybridizing different wheat–Th. intermediumand wheat–barley disomic alien substitution lines, with the aim of using genes inTh. intermediumand barley for wheat breeding and investigating the genetic behavior of alien chromosomes and their wheat homoeologs. As expected, we obtained two types of wheat double substitution lines, 2D2Ai#2(2B)2H(2A) and 2A2Ai#2(2B)2H(2D), in which different group 2 wheat chromosomes were replaced by barley chromosome 2H andTh. intermediumchromosome 2Ai#2. The new materials were characterized using molecular markers, genomicin situhybridization (GISH), and fluorescentin situhybridization (FISH). GISH and FISH experiments revealed that the double substitution lines harbor 42 chromosomes including 38 wheat chromosomes, a pair of barley chromosomes, and a pair ofTh. intermediumchromosomes. Analysis using specific DNA markers showed that two pairs of wheat homoeologous group 2 chromosomes in the new lines were substituted by a pair of 2H and a pair of 2Ai#2 chromosomes. Chromosome 2H showed a higher transmission rate than 2Ai#2, and both chromosomes were preferentially transmitted between generationsviafemale gametes. Evaluation of botanic and agronomic traits demonstrated that, compared with their parents, the new lines showed similar growth habits and plant type but differences in plant height, flowering date, and self-fertility. Cytological observations using different probes suggested that the double substitution lines showed nearly normal genetic behavior before and during meiosis. The novel substitution lines can potentially be used in wheat meiosis research and breeding programs.