In vitro cytotoxicity of solid epoxy-based dental resins and their components
In vitro cytotoxicity of solid epoxy-based dental resins and their components
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DOI：
10.1016/s0109-5641(99)00058-5
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发表时间：
1999-09-01
期刊：
影响因子：
5
通讯作者：
Yourtee, DM
中科院分区：
文献类型：
--
作者：
Kostoryz, EL;Tong, PY;Yourtee, DM
目的：本研究的目的是评估加入螺原碳酸酯（SOC）或多元醇对环氧基牙科树脂的细胞毒性的影响。方法：树脂含有环氧树脂之一：二缩水甘油醚双酚A（戈伊-6004）; 3，4-环氧环己烷甲基-3，4-环氧环己烷羧酸酯（UVR-6105）;乙烯基环己烷二氧化物（ERL-4206）或三环氧混合物（环氧-M）。SOC为t/t-2，3，8，9-二（四亚甲基）-1，5，7，11-四氧杂螺[5.5]十一烷（SOC）。多元醇是聚四氢呋喃（p-THF-250）和聚己内酯三醇（TONE-301）。光引发剂（4-辛基苯基）苯基碘六氟锑酸盐和邻苯二酚用于光固化树脂。结果：在琼脂扩散试验中，ERL-4206和UVR-6105树脂均表现出较强的细胞毒性（+），而SOC的加入使其变为无细胞毒性（-）。添加1-3% SOC将环氧-M从轻度（+）变为无细胞毒性。添加SOC后，戈伊-6004的细胞毒性从中度（+ +）变为轻度（-）。通常，当添加到多元醇/环氧树脂组合中时，添加SOC不会改变细胞毒性。当添加到UVR-6105中时，两种多元醇都会产生细胞毒性降低的树脂，但当添加到Epoxy-M树脂中时，情况恰恰相反。在MTT分析中，对来自100 μ l树脂提取物的细胞存活百分比进行统计学比较（ANOVA，p < 0.05）。环氧-M和戈伊-6004树脂浸提液的细胞毒性显著低于UVR-6105和ERL-4206树脂浸提液。总体而言，SOC组分降低了所有SOC/环氧树脂组合的细胞毒性，但SOC/ERL-4206除外，其细胞毒性显著高于ERL-4206树脂浸提液。这可能是在琼脂扩散分析中观察到SOC/ERL-4206的细胞固定作用的结果。与其非SOC对应物相比，添加SOC产生显著更少的细胞毒性SOC/多元醇/环氧-M树脂。用SOC/多元醇/UVR-6105树脂组合获得相反的结果。与琼脂扩散结果一致，添加多元醇显著降低了UVR-6105树脂的细胞毒性。这些树脂的细胞毒性可能与其组分作为沥滤物的50%细胞毒性（TC 50）有关。将各个组分的TC 50值与BISGMA进行比较。多元醇、环氧单体、SOC单体和邻苯二酚的细胞毒性显著低于BISGMA.Significance：在环氧基树脂的配方中加入SOC和多元醇可能有助于生物相容性牙科复合材料的开发。(C)1999年牙科材料学院。由爱思唯尔科技有限公司出版。保留所有权利。
Objective: The objective of this study was to evaluate the effect of adding a spiroorthocarbonate (SOC) or a polyol on the cytotoxicity of epoxy-based dental resins.Methods: Resins contained one of the epoxies: diglycidyl ether Bisphenol A (GY-6004); 3,4-epoxycyclohexanemethyl-3,4-epoxycyclohexane carboxylate (UVR-6105); vinyl cyclohexane dioxide (ERL-4206) or the three-epoxy mixture (Epoxy-M). The SOC was t/t-2,3,8,9-di(tetramethylene)-1,5,7,11-tetraoxaspiro[5.5]undecane (SOC). The polyols were polytetrahydrofuran (p-THF-250) and polycaprolactone triol (TONE-301). The photoinitiator (4-octylphenyl)phenyliodonium hexafluoroantimonate and camphorquinone were used for light curing the resins. Four types of resins (epoxy, SOC/epoxy, polyol/epoxy and SOC/polyol/epoxy) were evaluated for cytotoxicity as solids in the agar diffusion assay and as aqueous extracts in the MTT assay using L929 cells.Results: In agar diffusion analysis, ERL-4206 and UVR-6105 resins were severely cytotoxic (+ + +), but the addition of SOC changed them to non-cytotoxic (-). Addition of 1-3% SOC changed Epoxy-M from mild (+) to non-cytotoxic. Adding SOC changed GY-6004 from moderate (+ +) to mild (-) cytotoxicity. Generally, addition of SOC did not change cytotoxicity when added to polyol/epoxy combinations. Either polyol produced resins with reduced cytotoxicity when added to UVR-6105, but the opposite occurred when added to Epoxy-M resins. In MTT analysis, percent cell survival from 100 mu l resin extracts were statistically compared (ANOVA, p < 0.05). Epoxy-M and GY-6004 resin extracts were significantly less cytotoxic than UVR-6105 and ERL-4206 resin extracts were. Overall, the SOC component reduced the cytotoxicity of all SOC/epoxy combinations, except SOC/ERL-4206, which was significantly more cytotoxic than ERL-4206 resin extract. This may be the result of cell fixative effects observed for SOC/ERL-4206 in agar diffusion analysis. Addition of SOC produced significantly less cytotoxic SOC/polyol/Epoxy-M resins when compared to its non-SOC counterpart. The contrary result was obtained with SOC/polyol/UVR-6105 resin combinations. Consistent with agar diffusion results, adding polyol significantly decreased cytotoxicity of UVR-6105 resins. The cytotoxicity of these resins may be related to the 50% cytotoxicity (TC50) of their components as leachates. The TC50 values of the individual components were compared to BISGMA. Polyols, epoxy monomers, SOC monomer and camphorquinone were significantly(p < 0.05) less cytotoxic than BISGMA.Significance: Addition of SOCs and polyols in the formulation of epoxy-based resins may contribute to development of biocompatible dental composites. (C) 1999 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.