Design and characterization of calcium phosphate ceramic scaffolds for bone tissue engineering.
Design and characterization of calcium phosphate ceramic scaffolds for bone tissue engineering.
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DOI：
10.1016/j.dental.2015.09.008
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Kuhn LT
中科院分区：
文献类型：
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作者：
Denry I;Kuhn LT
我们的目标是根据磷酸钙陶瓷支架（CPS）在骨组织工程中的短暂作用以及有效骨再生的相关要求，回顾其制造的设计策略。我们研究了可满足 CPS 机械和生物要求的各种设计选项，随后重点关注在结构、机械性能和生物降解性等时间敏感特性方面正确表征 CPS 的重要性。最后，讨论了体外与体内测试之间的关系，试图强调可靠的性能预测因素。 CPS 应采用组合设计策略，考虑到 3D 架构、足够的表面化学和形貌，所有这些都是促进骨形成所必需的。 CPS 是输送成骨因子和抗感染药物的首选介质。非成骨细胞介导的矿物质沉积可能会干扰 CPS 的体外成骨测试，因此除了矿物质含量增加之外，还应确认包括 I 型胶原、骨唾液蛋白和骨钙素在内的多种蛋白质或基因的表达。 CPS 是一种优良的骨再生支架材料，因为它们能积极促进骨生成。 CPS 通过释放钙和磷酸盐实现生物降解，这是一种独特的优势。 CPS在宏观、微观和纳米尺度上的结构控制及其与细胞和聚合物材料的结合可能会导致骨组织工程的重大发展。
Our goal is to review design strategies for the fabrication of calcium phosphate ceramic scaffolds (CPS), in light of their transient role in bone tissue engineering and associated requirements for effective bone regeneration. We examine the various design options available to meet mechanical and biological requirements of CPS and later focus on the importance of proper characterization of CPS in terms of architecture, mechanical properties and time-sensitive properties such as biodegradability. Finally, relationships between in vitro vs. in vivo testing are addressed, with an attempt to highlight reliable performance predictors. A combinatory design strategy should be used with CPS taking into consideration 3D architecture, adequate surface chemistry and topography, all of which are needed to promote bone formation. CPS represent the media of choice for delivery of osteogenic factors and anti-infectives. Non-osteoblast mediated mineral deposition can confound in vitro osteogenesis testing of CPS and therefore the expression of a variety of proteins or genes including collagen type I, bone sialoprotein and osteocalcin should be confirmed in addition to increased mineral content. CPS are a superior scaffold material for bone regeneration because they actively promote osteogenesis. Biodegradability of CPS via calcium and phosphate release represents a unique asset. Structural control of CPS at the macro, micro and nanoscale and their combination with cells and polymeric materials is likely to lead to significant developments in bone tissue engineering.