Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter
Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter
复制标题
DOI：
10.1104/pp.105.069963
复制
发表时间：
2005-11-01
期刊：
影响因子：
7.4
通讯作者：
Jordano, J
中科院分区：
文献类型：
--
作者：
Díaz-Martín, J;Almoguera, CN;Jordano, J
Hahsp17.6G1是向日葵（Helianthus annuus）中一种小分子热应激蛋白（small heat stress protein，sHSP）的启动子，该蛋白在合子胚胎发生过程中被激活，但对热应激没有反应。我们在这里报告的转录因子（TF），向日葵干旱响应元件结合因子2（HaDREB 2），通过单杂交与功能的顺式元件在Hahsp17.6G1的相互作用的克隆。我们已经分析了HaDREB 2和第二个转录因子，向日葵热应激因子A9（HaHSFA 9），这是以前分配到Hahsp17.6G1的调节之间的功能相互作用。HaDREB 2和HaHSFA 9协同反式激活轰击向日葵胚中的Hahsp17.6G1启动子。这种协同相互作用是热应激因子（HSF）特异性的，并且需要两种因子与启动子结合。HaHSFA 9的C-末端区域足以用于HSF特异性。我们的研究结果代表了Apetala 2（HaDREB 2）和HSF（HaHSFA 9）转录因子家族成员之间的功能相互作用的一个例子。我们建议新的角色在合子胚胎发生的特定成员的AP 2转录因子家族。
Hahsp17.6G1 is the promoter of a small heat stress protein (sHSP) from sunflower (Helianthus annuus) that is activated during zygotic embryogenesis, but which does not respond to heat stress. We report here the cloning of a transcription factor (TF), sunflower drought-responsive element binding factor 2 (HaDREB2), by one-hybrid interaction with functional cis-elements in Hahsp17.6G1. We have analyzed the functional interaction between HaDREB2 and a second transcription factor, sunflower heat stress factor A9 (HaHSFA9), which was previously assigned to the regulation of Hahsp17.6G1. HaDREB2 and HaHSFA9 synergistically trans-activate the Hahsp17.6G1 promoter in bombarded sunflower embryos. This synergistic interaction is heat stress factor (HSF) specific and requires the binding of both factors to the promoter. The C-terminal region of HaHSFA9 is sufficient for the HSF specificity. Our results represent an example of a functional interaction between members of the Apetala 2 (HaDREB2) and HSF (HaHSFA9) families of transcription factors. We suggest new roles in zygotic embryogenesis for specific members of the AP2 transcription factor family.