Ion-dependent metabolic responses of Vicia faba L. to salt stress.
Ion-dependent metabolic responses of Vicia faba L. to salt stress.
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DOI：
10.1111/pce.13386
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发表时间：
2018-08
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影响因子：
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通讯作者：
J. Richter;J. Behr;A. Erban;J. Kopka;C. Zörb
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文献类型：
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作者：
J. Richter;J. Behr;A. Erban;J. Kopka;C. Zörb
受盐影响的农田越来越多地被钠、钙和镁的氯化物、碳酸盐和硫酸盐所负担。有趣的是，几乎总是在NaCl胁迫下研究潜在的生理过程。两个蚕豆品种进行低盐和高盐处理的NaCl，Na 2SO 4，和KCl。盐胁迫7天后，同化率和叶片水蒸气传导率下降到约25-30%，而生物量没有减少，但这并没有造成严重的碳短缺。Na+，K+，Cl-等摩尔处理表现出可比的积累模式，在叶和根中，除了SO 4 2-没有积累。为了详细了解测试离子组合引起的影响，我们进行了基于非靶向气相色谱-质谱的代谢物分析。对各种盐的代谢反应部分高度线性相关，但只有少数代谢物的反应是共同的所有盐和两个品种。在高盐浓度下，只有肌醇，尿囊素，和glycerophosphoglycerol非常显着增加根在所有测试条件下。我们发现了几种代谢反应，优先与Na+，K+，或Cl-的存在。例如，叶片脯氨酸含量的增加和叶片富马酸和苹果酸含量的减少与Cl-积累明显相关。
Salt-affected farmlands are increasingly burdened by chlorides, carbonates, and sulfates of sodium, calcium, and magnesium. Intriguingly, the underlying physiological processes are studied almost always under NaCl stress. Two faba bean cultivars were subjected to low- and high-salt treatments of NaCl, Na2 SO4 , and KCl. Assimilation rate and leaf water vapor conductance were reduced to approximately 25-30% without biomass reduction after 7 days salt stress, but this did not cause severe carbon shortage. The equimolar treatments of Na+ , K+ , and Cl- showed comparable accumulation patterns in leaves and roots, except for SO4 2- which did not accumulate. To gain a detailed understanding of the effects caused by the tested ion combinations, we performed nontargeted gas chromatography-mass spectrometry-based metabolite profiling. Metabolic responses to various salts were in part highly linearly correlated, but only a few metabolite responses were common to all salts and in both cultivars. At high salt concentrations, only myo-inositol, allantoin, and glycerophosphoglycerol were highly significantly increased in roots under all tested conditions. We discovered several metabolic responses that were preferentially associated with the presence of Na+ , K+ , or Cl- . For example, increases of leaf proline and decreases of leaf fumaric acid and malic acid were apparently associated with Cl- accumulation.