A microfluidic device for epigenomic profiling using 100 cells.
A microfluidic device for epigenomic profiling using 100 cells.
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DOI：
10.1038/nmeth.3488
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发表时间：
2015-10
期刊：
影响因子：
48
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Cao Z;Chen C;He B;Tan K;Lu C
染色质免疫沉淀（ChIP）检测的灵敏度是低丰度细胞表观基因组研究的主要障碍。在这里，我们提出了一种基于微流体的ChIP-Seq协议，通过大幅改善高质量ChIP富集DNA的收集，使用少至100个细胞。利用这项技术，我们发现了许多新的增强子和超级增强子在造血干细胞和祖细胞从小鼠胎肝，这表明增强子活性是高度动态的早期造血。
The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-Seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many novel enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.