Cell tracing reveals a dorsoventral lineage restriction plane in the mouse limb bud mesenchyme
Cell tracing reveals a dorsoventral lineage restriction plane in the mouse limb bud mesenchyme
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DOI：
10.1242/dev.02873
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发表时间：
2007-10-15
期刊：
影响因子：
4.6
通讯作者：
Torres, Miguel
中科院分区：
文献类型：
--
作者：
Arques, Carlos G.;Doohan, Roisin;Torres, Miguel
区域化的胚胎领域成为独立的单位的增长和模式是一个普遍的战略在后生动物的发展。commonly代表了这种区域化的一个特殊实例，其中相邻区域之间的细胞谱系限制保持了单位的一致性。在昆虫和脊椎动物的发育过程中已经描述了谱系区室。到目前为止所描述的隔室的两个共同特征是它们在上皮结构中的发生和隔室边界处的信号传导区域的存在。尽管果蝇隔室组织代表胚胎领域的背景细分，其不一定与解剖结构相关，但迄今为止描述的脊椎动物隔室边界与解剖或细胞类型的不连续性一致或预期。在这里，我们描述了一个通用的方法，在小鼠克隆分析，并使用它来确定克隆分布的拓扑结构沿着三个肢体轴。我们确定了一个谱系限制边界在肢体间充质背腹侧边界是无关的任何解剖不连续性，其谱系限制边界是没有明显的任何信号中心。这种限制是脊椎动物中第一个与解剖学边界无关的原基细分机制的例子。此外，这是在任何生物体中的间充质结构内描述的第一个谱系区室，表明谱系限制不仅对上皮结构，而且对间充质场模式化都是基本的。没有发现谱系划分沿沿着proximodistal或前后轴，表明图案沿着这些轴不涉及限制细胞分散在特定的轴向位置。
Regionalization of embryonic fields into independent units of growth and patterning is a widespread strategy during metazoan development. Compartments represent a particular instance of this regionalization, in which unit coherence is maintained by cell lineage restriction between adjacent regions. Lineage compartments have been described during insect and vertebrate development. Two common characteristics of the compartments described so far are their occurrence in epithelial structures and the presence of signaling regions at compartment borders. Whereas Drosophila compartmental organization represents a background subdivision of embryonic fields that is not necessarily related to anatomical structures, vertebrate compartment borders described thus far coincide with, or anticipate, anatomical or cell- type discontinuities. Here, we describe a general method for clonal analysis in the mouse and use it to determine the topology of clone distribution along the three limb axes. We identify a lineage restriction boundary at the limb mesenchyme dorsoventral border that is unrelated to any anatomical discontinuity, and whose lineage restriction border is not obviously associated with any signaling center. This restriction is the first example in vertebrates of a mechanism of primordium subdivision unrelated to anatomical boundaries. Furthermore, this is the first lineage compartment described within a mesenchymal structure in any organism, suggesting that lineage restrictions are fundamental not only for epithelial structures, but also for mesenchymal field patterning. No lineage compartmentalization was found along the proximodistal or anteroposterior axes, indicating that patterning along these axes does not involve restriction of cell dispersion at specific axial positions.