Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
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DOI：
10.1038/srep19032
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发表时间：
2016-01-08
影响因子：
4.6
通讯作者：
Barnich N
中科院分区：
文献类型：
--
作者：
Agus A;Denizot J;Thévenot J;Martinez-Medina M;Massier S;Sauvanet P;Bernalier-Donadille A;Denis S;Hofman P;Bonnet R;Billard E;Barnich N
最近的进展表明，在CD中观察到的异常炎症反应涉及肠道微生物群、宿主遗传学和环境因素之间的相互作用。在西方国家，脂肪和糖的消费量不断增加，这与世纪后期CD发病率的增加相平行。评价HF/HS饮食对小鼠肠道微炎症、肠道微生物群组成、功能和E.大肠杆菌群。HF/HS饮食在肠道中创造了一个特定的炎症环境，与肠粘膜生态失调相关，其特征在于促炎变形菌门（如E.大肠杆菌，保护性细菌减少，SCFA浓度显著降低。与对照组相比，用HF/HS饮食处理的小鼠中GPR 43（SCFA受体）的表达降低，并且CD患者中GPR 43的表达降低。有趣的是，用GPR 43激动剂处理的小鼠被保护免于DSS诱导的结肠炎。最后，HF/HS处理小鼠的粪便移植到GF小鼠增加了对AIEC感染的易感性。总之，我们的研究结果表明，西方饮食可以加重炎症过程，GPR 43受体途径的激活可以用作治疗CD患者的新策略。
Recent advances have shown that the abnormal inflammatory response observed in CD involves an interplay among intestinal microbiota, host genetics and environmental factors. The escalating consumption of fat and sugar in Western countries parallels an increased incidence of CD during the latter 20th century. The impact of a HF/HS diet in mice was evaluated for the gut micro-inflammation, intestinal microbiota composition, function and selection of an E. coli population. The HF/HS diet created a specific inflammatory environment in the gut, correlated with intestinal mucosa dysbiosis characterized by an overgrowth of pro-inflammatory Proteobacteria such as E. coli, a decrease in protective bacteria, and a significantly decreased of SCFA concentrations. The expression of GPR43, a SCFA receptor was reduced in mice treated with a HF/HS diet and reduced in CD patients compared with controls. Interestingly, mice treated with an agonist of GPR43 were protected against DSS-induced colitis. Finally, the transplantation of feces from HF/HS treated mice to GF mice increased susceptibility to AIEC infection. Together, our results demonstrate that a Western diet could aggravate the inflammatory process and that the activation of the GPR43 receptor pathway could be used as a new strategy to treat CD patients.