Synthesis in vitro of 5,6-dihydroxyindole-2-carboxylic acid by dopachrome conversion factor from Cloudman S91 melanoma cells.
Synthesis in vitro of 5,6-dihydroxyindole-2-carboxylic acid by dopachrome conversion factor from Cloudman S91 melanoma cells.
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通过 Cloudman S91 黑色素瘤细胞的多巴色素转换因子体外合成 5,6-二羟基吲哚-2-羧酸。
DOI：
10.1111/1523-1747.ep12276688
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Gettins,P
中科院分区：
文献类型：
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作者：
Korner,AM;Gettins,P
多巴色素氧化成黑色素在体外自发发生，但已显示在体内受酶控制。从Cloudman S91黑色素瘤细胞纯化的多巴色素转化因子将橙子的多巴色素转化为无色化合物。我们对该无色化合物进行了纯化，并鉴定其为5，6-二羟基吲哚-2-羧酸。该化合物由含有多巴和多巴色素的溶液酶促产生，并且在这些条件下，在pH 6.8的溶液中不易氧化成黑色素。5，6-二羟基吲哚-2-羧酸的体外合成表明，该化合物可能是黑色素合成的中间体。
The oxidation of dopachrome to melanin occurs spontaneously in vitro but has been shown to be under enzymatic control in vivo. Dopachrome conversion factor, purified from Cloudman S91 melanoma cells, converts dopachrome, which is orange, to a colorless compound. We have purified this colorless compound and identified it as 5,6-dihydroxyindole-2-carboxylic acid. This compound is generated enzymatically from a solution containing dopa and dopachrome and, under these conditions, is not readily oxidized to melanin in solution at pH 6.8. The synthesis in vitro of 5,6-dihydroxyindole- 2-carboxylic acid suggests that this compound may be an intermediate in the synthesis of melanin.