Expression and Localization of α-amylase in the Submandibular and Sublingual Glands of Mice.
Expression and Localization of α-amylase in the Submandibular and Sublingual Glands of Mice.
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DOI：
10.1267/ahc.14005
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发表时间：
2014-06-28
影响因子：
2.4
通讯作者：
Iseki S
中科院分区：
文献类型：
--
作者：
Yamagishi R;Wakayama T;Nakata H;Adthapanyawanich K;Kumchantuek T;Yamamoto M;Iseki S
在小鼠的主要唾液腺中，腮腺（PG）中的腺泡细胞已知是产生α-淀粉酶的主要部位，而下颌骨腺（SMG）和舌下腺（SLG）中α-淀粉酶的产生以及负责α-淀粉酶产生的细胞类型尚未确定。为了澄清这一问题，我们采用定量和组织化学方法检测了α-淀粉酶mRNA和蛋白在雄性和雌性小鼠大唾液腺中的表达和定位。α-淀粉酶mRNA水平依次为PG、SMG、SLG。PG和SLG中α-淀粉酶mRNA水平无性别差异，而雌性SMG中α-淀粉酶mRNA水平约为雄性SMG的30%。原位杂交和免疫组化检测结果显示，α-淀粉酶mRNA和蛋白在雄性SMG的PG、SLG的浆液半乳细胞和颗粒曲小管（GCT）细胞中呈强阳性，在雌雄SMG的浆液腺泡细胞中呈弱阳性，在SLG的粘液腺泡细胞中呈阴性。这些结果表明，α-淀粉酶主要由GCT细胞产生，部分由SMG中的腺泡细胞产生，而在小鼠SLG中，α-淀粉酶仅由浆液半乳细胞产生。
In the major salivary glands of mice, acinar cells in the parotid gland (PG) are known to be the main site for the production of the digestive enzyme α-amylase, whereas α-amylase production in the submandibular gland (SMG) and sublingual gland (SLG), as well as the cell types responsible for α-amylase production, has been less firmly established. To clarify this issue, we examined the expression and localization of both the mRNA and protein of α-amylase in the major salivary glands of male and female mice by quantitative and histochemical methods. α-amylase mRNA levels were higher in the order of PG, SMG, and SLG. No sexual difference was observed in α-amylase mRNA levels in the PG and SLG, whereas α-amylase mRNA levels in the female SMG were approximately 30% those in the male SMG. Using in situ hybridization and immunohistochemistry, signals for α-amylase mRNA and protein were found to be strongly positive in acinar cells of the PG, serous demilune cells of the SLG, and granular convoluted tubule (GCT) cells of the male SMG, weakly positive in seromucous acinar cells of the male and female SMG, and negative in mucous acinar cells of the SLG. These results clarified that α-amylase is produced mainly by GCT cells and partly by acinar cells in the SMG, whereas it is produced exclusively by serous demilune cells in the SLG of mice.
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影响因子：
3.2
作者：
Morley, Debra J.;Hodes, M. E.
通讯作者：
Hodes, M. E.
影响因子：
3
作者：
KRAUS, FW;MESTECKY, J
通讯作者：
MESTECKY, J
DOI：
10.1016/s0074-7696(08)61759-3
发表时间：
1980-01-01
期刊：
International review of cytology
影响因子：
--
作者：
Pinkstaff, C A
通讯作者：
Pinkstaff, C A
影响因子：
2.4
作者：
Nakata H;Wakayama T;Adthapanyawanich K;Nishiuchi T;Murakami Y;Takai Y;Iseki S
通讯作者：
Iseki S
影响因子：
64.5
作者：
HAGENBUCHLE, O;BOVEY, R;YOUNG, RA
通讯作者：
YOUNG, RA