Development of a sensitive and rapid UHPLC-MS/MS method for simultaneous quantification of nine compounds in rat plasma and application in a comparative pharmacokinetic study after oral administration of Xuefu Zhuyu Decoction and nimodipine
Development of a sensitive and rapid UHPLC-MS/MS method for simultaneous quantification of nine compounds in rat plasma and application in a comparative pharmacokinetic study after oral administration of Xuefu Zhuyu Decoction and nimodipine
复制标题
建立灵敏快速的 UHPLC-MS/MS 方法同时定量大鼠血浆中 9 种化合物，并应用于口服血府逐瘀汤和尼莫地平后的药代动力学比较研究
DOI：
10.1002/bmc.4872
复制
发表时间：
2020-07-16
影响因子：
1.8
通讯作者：
Huang, Gang
中科院分区：
文献类型：
--
作者：
Fu, Chunyan;Wu, Qian;Huang, Gang
血瘀专瘀汤（XFZYD）是一种用于临床治疗创伤性脑损伤（TBI）的中药方剂。建立一种灵敏、快速的高效液相色谱-质谱联用方法，同时研究尼莫地平和XFZYD中苦杏仁苷、羟基红花黄a、芦丁、柳枝苷、narnarutin、柚皮苷、新橙皮苷和柴胡皂苷a在颅脑损伤大鼠体内的药动学。多反应监测对9种分析物和内标物的检测选择性高，无明显干扰。在较宽的浓度范围内，校准曲线呈良好的线性关系（r > 0.99）。9种分析物的平均绝对回收率为85 ~ 106%，基质效应均在80 ~ 120%范围内。日内、日间精密度和准确度均可接受(RSD，
Xuefu Zhuyu Decoction (XFZYD) is a traditional Chinese medicine prescription used for the clinical treatment of traumatic brain injury (TBI). The purpose of this work was to develop a sensitive and rapid UHPLC-MS/MS method to simultaneously study the pharmacokinetics of nimodipine and eight components of XFZYD, namely, amygdalin, hydroxysafflor yellow A, rutin, liquiritin, narirutin, naringin, neohesperidin and saikosaponin A, in rats with and without TBI. Multiple reaction monitoring was highly selective in the detection of nine analytes and the internal standard without obvious interference. The calibration curves displayed good linearity (r > 0.99) over a wide concentration range. The mean absolute recoveries of the nine analytes were 85-106%, and all matrix effects were in the range 80-120%. The intra- and inter-day precision and accuracy were acceptable (RSD,