Developmental regulation of notochord-derived repulsion for dorsal root ganglion axons
Developmental regulation of notochord-derived repulsion for dorsal root ganglion axons
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DOI：
10.1016/j.mcn.2003.10.005
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发表时间：
2004-02-01
影响因子：
3.5
通讯作者：
Shiga, T
中科院分区：
文献类型：
--
作者：
Masuda, T;Fukamauchi, F;Shiga, T
在发育的初始阶段，脊索通过semaphorin 3A/neuropilin-1、axonin-1/SC2和其他未知的排斥分子为背根神经节（DRG）轴突提供排斥信号。已知脊索产生聚集蛋白聚糖，其为硫酸软骨素蛋白聚糖（CSPG）之一。我们在此报道，在培养基中加入聚集蛋白聚糖不仅可以诱导DRG生长锥塌陷，而且可以抑制DRG轴突生长。使用来自鸡胚或neuropilin-l-缺陷的小鼠软骨素酶ABC处理的组织组成的共培养物，我们显示的直接证据表明，CSPGs参与脊索源性排斥DRG轴突。在以后的发展阶段，CSPGs参与perinotochordal鞘衍生的轴突排斥，但不是在脊索核心衍生的排斥。我们进一步证明，TAG-1/axonin-1/SC2不参与介导CSPGs的排斥活动，但脊索核心衍生的轴突排斥所需的。因此，脊索衍生的多个轴突排斥以时空特异性的方式作用，以形成DRG轴突的初始轨迹。(C)2003年爱思唯尔公司All rights reserved.
During the initial stages of development, the notochord provides repulsive signals for dorsal root ganglion (DRG) axons via semaphorin 3A/neuropilin-1, axonin-1/SC2, and other unknown repulsive molecules. The notochord is known to produce aggrecan, one of the chondroitin sulfate proteoglycans (CSPGs). We report here that adding aggrecan to the culture medium cannot only induce DRG growth cone collapse, but also inhibit DRG axonal growth. Using cocultures composed of tissues derived from chick embryos or neuropilin-l-deficient mice treated with chondroitinase ABC, we show the direct evidence that CSPGs are involved in notochord-derived repulsion for DRG axons. At later developmental stages, CSPGs are involved in perinotochordal sheath-derived axon repulsion, but not in notochord core-derived repulsion. We further demonstrate that TAG-1/axonin-1/SC2 is not involved in mediating repulsive activities by CSPGs, but is required for notochord core-derived axon repulsion. Thus, notochord-derived multiple axon repulsions act in a spatiotemporal-specific manner to shape the initial trajectories of DRG axons. (C) 2003 Elsevier Inc. All rights reserved.