Role of thymulin on the somatotropic axis in vivo.
Role of thymulin on the somatotropic axis in vivo.
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DOI：
10.1016/j.lfs.2012.06.037
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发表时间：
2012-09
期刊：
影响因子：
6.1
通讯作者：
P. Reggiani;E. Martines;G. Camihort;Brenda Poch;R. Goya;G. Cónsole
中科院分区：
文献类型：
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作者：
P. Reggiani;E. Martines;G. Camihort;Brenda Poch;R. Goya;G. Cónsole
目的有明确的证据表明存在双向胸腺-促生长轴，一些研究表明，胸腺肽胸腺肽可能参与这种沟通。本研究采用C57 BL/6小鼠血清胸腺素免疫中和及新生胸腺素基因治疗（NTGT）裸鼠，观察其对生长激素细胞的增重及形态计量学的影响。在控制。使用表达胸腺肽合成基因的腺病毒载体（RAd-FTS）在裸鼠中实施NTGT。在出生后第1天，杂合（nu/+）和纯合（nu/nu）幼仔接受单次双侧i.m.注射RAd-FTS或RAd-GFP（表达绿色荧光蛋白的对照载体）。记录体重增加，并在研究结束时对垂体进行生长激素（GH）免疫染色。血清生长激素和胸腺肽测定放射免疫法和bioassays.KEY发现胸腺肽免疫中和引起体重增加，血清生长激素和生长激素细胞密度的显着下降，以及生长激素细胞的大小增加。NTGT能显著增加nu/nu小鼠的体重增长、血清胸腺素（P<0.01）、生长激素细胞和体密度。
AIMSThere is clear evidence for the existence of a bi-directional thymus–somatotropic axis and several studies suggest that the thymic peptide thymulin may be involved in this communication. We undertook to assess the impact of serum thymulin immunoneutralization in C57BL/6 mice and that of neonatal thymulin gene therapy (NTGT) in nude mice on body weight (BW) gain and on the histomorphometric profile of the somatotrope population.MAIN METHODSImmunoneutralization of thymulin was done from postnatal day 1 to 35 by i.p. injections of rabbit anti-thymulin serum (α-FTS) and normal rabbit serum (NRS) in controls. NTGT was implemented in nudes using an adenoviral vector expressing a synthetic gene for thymulin (RAd-FTS). On postnatal day 1, heterozygous (nu/+) and homozygous (nu/nu) pups received a single bilateral i.m. injection either RAd-FTS or RAd-GFP (a control vector expressing green fluorescent protein). BW gain was recorded and at the end of the study the pituitaries were immunostained for growth hormone (GH). Serum GH and thymulin were determined by radioimmunoassay and bioassay, respectively.KEY FINDINGSThymulin immunoneutralization induced a significant decrease in BW gain, serum GH and somatotrope cell density as well as an increase in somatotrope cell size. NTGT markedly increased BW gain, serum thymulin (P<0.01) and somatotrope cell and volume density in nu/nu mice.SIGNIFICANCEOur results suggest that thymulin plays a relevant physiological role on the thymus–somatotropic axis in mice.