193-nm photodissociation of singly and multiply charged peptide anions for acidic proteome characterization.
193-nm photodissociation of singly and multiply charged peptide anions for acidic proteome characterization.
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DOI：
10.1002/pmic.201000565
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发表时间：
2011-04
期刊：
影响因子：
3.4
通讯作者：
Brodbelt, Jennifer S.
中科院分区：
文献类型：
--
作者：
Madsen, James A.;Kaoud, Tamer S.;Dalby, Kevin N.;Brodbelt, Jennifer S.
利用193 nm紫外光解离(UVPD)对单电荷和多电荷多肽阴离子进行了测序。用这种方法解离后，a-/x型，其次是d和w侧链损失离子，是最多和最丰富的序列离子，通常产生100%的序列覆盖率。单电荷和多电荷阴离子的离解行为明显不同，较高的电荷前体产生更多的序列离子；然而，所研究的所有电荷状态(1-到3-)都产生了丰富的诊断信息。193 nm处的UVPD也被证明能够成功地区分和精确定位不稳定的磷酸化修饰。序列离子的产生具有很高的丰度，需要有限的平均才能获得满意的光谱质量。紫外光激发产生的完整的、电荷还原的自由基产物也受到碰撞诱导解离(称为激活电子光解离(a-EPD))的影响，但UVPD单独产生更可预测和更高丰度的序列离子。在碱性条件下(pH~11.5)，采用哌啶修饰的LC-MS/UVPD流动相，利用超快激活时间(5纳秒)成功地分析了丝裂原活化途径激酶(MAPKs)。
193 nm ultraviolet photodissociation (UVPD) was implemented to sequence singly and multiply charged peptide anions. Upon dissociation by this method, a-/x-type, followed by d and w side-chain loss ions, were the most prolific and abundant sequence ions, often yielding 100% sequence coverage. The dissociation behavior of singly and multiply charged anions was significantly different with higher charged precursors yielding more sequence ions; however, all charge states investigated (1- through 3-) produced rich diagnostic information. UVPD at 193 nm was also shown to successfully differentiate and pinpoint labile phosphorylation modifications. The sequence ions were produced with high abundances, requiring limited averaging for satisfactory spectral quality. The intact, charge-reduced radical products generated by UV photoexcitation were also subjected to collision induced dissociation (termed, activated – electron photodetachment dissociation (a-EPD)), but UVPD alone yielded more predictable and higher abundance sequence ions. With the use of a basic (pH ~11.5), piperidine-modified mobile phase, LC-MS/UVPD was implemented and resulted in the successful analysis of mitogen-activated pathway kinases (MAPKs) using ultrafast activation times (5 nanoseconds).
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影响因子：
4.4
作者：
Madsen JA;Boutz DR;Brodbelt JS
通讯作者：
Brodbelt JS
影响因子：
7.4
作者：
Zhang, Liangyi;Reilly, James P.
通讯作者：
Reilly, James P.
影响因子：
4.3
作者：
Kjeldsen, F;Silivra, OA;Zubarev, RA
通讯作者：
Zubarev, RA
DOI：
10.1016/j.jasms.2008.04.031
发表时间：
2008-08-01
影响因子：
3.2
作者：
Kjeldsen, Frank;Horning, Ole B.;Jensen, Ole N.
通讯作者：
Jensen, Ole N.
DOI：
10.1016/j.jasms.2010.01.015
发表时间：
2010-04-01
影响因子：
3.2
作者：
Larraillet, Vincent;Vorobyev, Aleksey;Dugourd, Philippe
通讯作者：
Dugourd, Philippe