Activation of lipolysis and cyclic AMP accumulation in rabbit adipocytes by isoproterenol in the presence of forskolin or pertussis toxin.
Activation of lipolysis and cyclic AMP accumulation in rabbit adipocytes by isoproterenol in the presence of forskolin or pertussis toxin.
复制标题
在毛喉素或百日咳毒素存在的情况下，异丙肾上腺素可激活兔脂肪细胞中的脂肪分解和环 AMP 积累。
DOI：
10.1016/0304-4165(84)90113-2
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
O'Donnell,CJ
中科院分区：
文献类型：
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作者：
Fain,JN;Garcia-Sainz,JA;Mills,I;O'Donnell,CJ
兔脂肪细胞对儿茶酚胺和毛喉素相对不敏感。然而，结合儿茶酚胺加毛喉素增加环AMP的积累和脂解比单独使用任何一种药物。百日咳毒素治疗也恢复了对儿茶酚胺的敏感性。在GTP存在下孵育的隔离膜中，没有发现腺苷酸环化酶的儿茶酚胺激活缺陷。兔脂肪细胞似乎有过量的抑制性鸟嘌呤核苷酸结合蛋白（Ni）。然而，在质膜中，这种蛋白质似乎是相对不活跃的，因为在GTP的存在下，儿茶酚胺激活腺苷酸环化酶活性。这些数据表明，在完整的兔脂肪细胞中，由于过量的抑制性鸟嘌呤核苷酸结合蛋白，儿茶酚胺和毛喉素作为腺苷酸环化酶的刺激剂是无效的。
Adipocytes from rabbits are relatively insensitive to catecholamines or forskolin. However, the combination of catecholamines plus forskolin increased cyclic AMP accumulation and lipolysis much more than either agent alone. Pertussis toxin treatment also restored sensitivity to catecholamines. No defect in activation by catecholamines of adenylate cyclase was seen in isolated membranes incubated in the presence of GTP. Rabbit adipocytes appear to have an excess of the inhibitory guanine nucleotide binding protein (Ni). However, in plasma membranes this protein appeared to be relatively inactive as there was an activation of adenylate cyclase activity by catecholamines in the presence of GTP. These data suggest that in intact rabbit adipocytes catecholamines and forskolin are ineffective as stimulators of adenylate cyclase due to an excess of inhibitory guanine nucleotide binding proteins.