Design of Biointerfaces for Regenerative Medicine
Design of Biointerfaces for Regenerative Medicine
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DOI：
10.1007/12_2011_161
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发表时间：
2011
影响因子：
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通讯作者：
Yusuke Arima;Koichi Kato;Koichi Kato;Y. Teramura;Y. Teramura;H. Iwata
中科院分区：
文献类型：
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作者：
Yusuke Arima;Koichi Kato;Koichi Kato;Y. Teramura;Y. Teramura;H. Iwata
了解和控制对人造材料的生物反应对于医疗器械和治疗的发展非常重要。链烷硫醇的自组装单分子层（SAM）提供了明确的表面，可以通过改变末端官能团来操纵。因此，自组装膜已被广泛用作研究人工材料如何影响生物反应的平台。在这里，我们回顾了细胞粘附行为的响应与各种表面特性的自组装膜和吸附的蛋白质对随后的细胞粘附的影响。我们还描述了一个应用程序的SAM作为培养神经干细胞（NSC）的基板。诱导固定化生长因子（如表皮生长因子）的正确取向的底物改善了细胞扩增期间纯NSC群体的选择。此外，我们还回顾了使用两亲聚合物修饰细胞和组织表面的新方法。用可以捕获和固定生物活性物质或细胞的两亲性聚合物涂覆细胞表面代表了用于临床应用，特别是细胞治疗的有前景的方法。
Understanding and controlling biological responses against artificial materials is important for the development of medical devices and therapies. Self-assembled monolayers (SAMs) of alkanethiols provide well-defined surfaces that can be manipulated by varying the terminal functional groups. Thus, SAMs have been extensively used as a platform for studying how artificial materials affect biological responses. Here, we review cell adhesion behavior in response to SAMs with various surface properties and the effects that adsorbed proteins have on subsequent cell adhesion. We also describe an application for SAMs as a substrate for culturing neural stem cells (NSCs). Substrates that induced the correct orientation of immobilized growth factors, like epidermal growth factor, improved the selection of a pure NSC population during cell expansion. In addition, we review new methodologies for using amphiphilic polymers to modify the surfaces of cells and tissues. Coating the cell surface with amphiphilic polymers that can capture and immobilize bioactive substances or cells represents a promising approach for clinical applications, particularly cellular therapies.