Immunoglobulin gene 'remnant' DNA--implications for antibody gene recombination.
Immunoglobulin gene 'remnant' DNA--implications for antibody gene recombination.
复制标题
免疫球蛋白基因“残余”DNA——对抗体基因重组的影响。
DOI：
10.1093/nar/12.10.4229
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发表时间：
1984
影响因子：
14.9
通讯作者：
Storb,U
中科院分区：
文献类型：
--
作者：
Selsing,E;Voss,J;Storb,U
在种系中，许多免疫球蛋白（Ig）产生细胞保留分离Ig可变区(V)和恒定区(C)基因的DNA。这个“残余”DNA必须在重组过程中移动，通过连接(J)片段连接V和C基因。我们分析了几种产生igg的细胞系中的残余dna。kappa （κ）轻链残余dna的核苷酸序列表明与V-J连接密切相关。我们在5个残余dna中发现了融合的Vκ和Jκ识别序列，这表明它们与V-J连接产生的融合的Vκ和Jκ片段之间存在相互关系。然而，在分析的16个浆细胞瘤残留dna中，均仅涉及与jκ 1的重组。因此，在大多数细胞系中，残余dna不能直接与重组的κ-基因相互作用。另一方面，我们对髓细胞的分析确实表明了残余dna和κ-基因之间的间接关系。我们的研究结果表明，在igg基因重排过程中，DNA重组发生了多个步骤。由于残余DNA连接位点没有表现出在igg基因V-J连接中观察到的灵活性，我们的研究结果还表明，连接机制可能涉及内切酶、外切酶和脂肪酶活性。
Many immunoglobulin (Ig)-producinq cells retain the DNA that separates Ig variable (V) and constant (C) region genes in the germline. This “remnant” DNA must be moved during the recombination process that joins V and C genes via a joining (J) segment. We have analyzed remnant DNAs in several Ig-producing cell lines. The nucleotide sequences of kappa (κ) light chain remnant DNAs indicate close relationships to V-J joining. We find fused Vκ and Jκrecognition sequencesin five remnant DNAs, suggesting reciprocal relationships to the fused Vκ and Jκ segments produced by V-J joining. However, of sixteen plasmacytoma remnant DNAs analyzed, all involve only recombination with Jκl. Thus, in most cell lines, remnant DNAs are notdirectlyreciprocal to recombined κ-genes. On the other hand, our analyses of myelotnas do indicate indirect relationships between remnant DNAs and κ-genes. Our results suggest that multiple steps of DNA recombination occur during Ig-gene rearrangement. Because remnant DNA joining sites do not exhibit the flexibility that has been observed in Ig-gene V-J joining, our findings also suggest that the joining mechanism may involve endonuclease, exonuclease and liqase activities.