Separation of exenatide analogue mono-PEGylated with 40 kDA polyethylene glycol by cation exchange chromatography
Separation of exenatide analogue mono-PEGylated with 40 kDA polyethylene glycol by cation exchange chromatography
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DOI：
10.1016/j.chroma.2011.07.107
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发表时间：
2011-09-28
影响因子：
4.1
通讯作者：
Yue, Peng
中科院分区：
文献类型：
--
作者：
Cai, Yongqing;Yue, Peng
随机聚乙二醇化通常产生由单聚乙二醇化异构体和多聚乙二醇化附件组成的产物混合物。通常在聚乙二醇化研究中，单聚乙二醇化异构体的分离是找到最佳聚乙二醇化位点的先决条件。然而，当肽或蛋白质用大至40 kDA的聚乙二醇聚乙二醇化时，异构体的物理化学性质如疏水性和分子大小将变得过于相似，使得常规分离方法如RP-HPLC、尺寸排阻色谱、SOS-PAGE和毛细管等电聚焦无效。本文介绍了一种用阳离子交换色谱法成功分离40 kDA聚乙二醇单聚乙二醇化的艾塞那肽类似物（一种治疗糖尿病的肠促胰岛素）异构体的方法，为聚乙二醇化研究提供了有力的工具。(C)2011爱思唯尔有限公司版权所有。
Random PEGylation usually resulted in product mixtures composed of mono-PEGylated isomers and multie-PEGylated attachments. Generally in PEGylation research, separation of the mono-PEGylated isomers was the prerequisite for finding the optimal PEGylation site. However, when peptides or proteins were PEGylated with polyethylene glycol as large as 40 kDA, the physicochemical properties like hydrophobicity and molecular size of the isomers would become too similar to make the routine separation methods, like RP-HPLC, size-exclusion chromatography, SOS-PAGE and capillary isoelectric focusing invalid. This article presented a useful method of successfully separating exenatide analogue (an incretin for diabetic therapy) isomers mono-PEGylated with 40 kDA polyethylene glycol by cation exchange chromatography, which would be a powerful tool for the PEGylation research. (C) 2011 Elsevier B.V. All rights reserved.