A New Colorimetric Platform for Protein Detection Based on Recognition-Induced Cascade of Polymeric Nanoparticles Disassembly
A New Colorimetric Platform for Protein Detection Based on Recognition-Induced Cascade of Polymeric Nanoparticles Disassembly
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基于识别诱导的聚合物纳米颗粒分解级联的新型蛋白质检测比色平台
DOI：
10.1002/mabi.201700392
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发表时间：
2018
影响因子：
4.6
通讯作者：
Yang Jing
中科院分区：
文献类型：
--
作者：
Fang Fang;Liu Jin;Li Yushu;Yang Junjiao;Yang Jing
尽管取得了很大的进展，但探索新的均相分析方法用于简单、直观和选择性的蛋白质检测仍然是人们高度感兴趣的。本文以聚合物胶束为传感支架，以蛋白质与聚合物胶束表面配体之间的分子识别为驱动力，触发检测信号的读出，研制了一种新型的蛋白质视觉检测比色传感器。通过将茜素红S(ARS)引入到疏水核中，使两亲性嵌段聚合物生物素标记的聚乙二醇-嵌段-聚(3-丙烯氨基苯基硼酸)自组装形成的聚合物胶束具有五颜六色的特征。基于对链霉亲和素的识别反应，这些胶束通过α-环糊精与硼酸的竞争结合进一步解离ARS，在2 h内实现橙黄色到粉红色-紫色的转变，这将为开发一种新的混合测量、直观、均一的分析方法开辟道路。
Despite great progress, it is still of high interest to explore new homogeneous assays for simple, visual, and selective protein detection. Herein, one new colorimetric sensor has been developed for visual detection of protein by using polymeric micelles as a sensing scaffold and the molecular recognition between protein and the ligand on the surface of the polymeric micelles as the driving force to trigger the readout of the detection signal. The polymeric micelles formed via the self‐assembly of the amphiphilic block polymer biotin‐labeled poly(ethylene glycol)‐block‐poly(3‐acryl aminophenylboronic acid) are endowed with colorful feature by incorporation of alizarin red S (ARS) into the hydrophobic core. Based on the response to streptavidin recognition, these micelles are further disintegrated through the competitive binding of α‐cyclodextrin with boronic acid for disassociation of ARS, which achieves orange–yellow to pink–purple transition in 2 h. This work will open the way to develop one new mix‐and‐measure, visual, and homogeneous assay.