Colon microflora in infants fed formula with galacto- and fructo-oligosaccharides:: More like breast-fed infants
Colon microflora in infants fed formula with galacto- and fructo-oligosaccharides:: More like breast-fed infants
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DOI：
10.1097/00005176-200501000-00007
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Wells, J
中科院分区：
文献类型：
--
作者：
Knol, J;Scholtens, P;Wells, J
目的：母乳喂养婴儿肠道菌群以双歧杆菌为主。我们的目的是研究添加了半乳寡糖和果寡糖（GOS/FOS）的婴儿配方奶粉是否能够建立两歧显性菌群，不仅在数量上，而且在结肠的代谢活性方面。方法：采用随机、双盲、安慰剂对照的干预研究方法，对两组婴儿进行评价，一组婴儿配方奶粉中GOS/FOS的比例为0.8 g/100 ml （OSF组），另一组喂食对照配方奶粉（SF组）。同时对母乳喂养组进行了研究。在研究开始时、4周和6周后，对粪便样本进行双歧杆菌数量、pH值、短链脂肪酸和乳酸盐的检测。结果：6周后，OSF组双歧杆菌的平均比例显著高于SF组（59.6%比49.5%,P < 0.05）。与对照组相比，OSF组婴儿的粪便平均pH值较低，醋酸盐比例升高，丙酸盐比例降低。OSF组和SF组的平均pH分别为5.7和6.3 （P < 0.001）。结论：在婴儿配方奶粉中添加益生元GOS/FOS混合物对双歧杆菌的生长和肠道总菌群的代谢活性有促进作用。益生元组中短链脂肪酸、乳酸盐和pH值的变化表明，与喂养对照配方奶粉的婴儿相比，母乳喂养婴儿的发酵情况更接近。
Objectives: The intestinal flora of breast-fed infants is generally dominated by Bifidobacteria. We aimed to investigate whether an infant formula supplemented with galacto-oligosaccharides and fructo-oligosaccharides (GOS/FOS) is able to establish a bifido-dominant microflora, not only in numbers but also with respect to the metabolic activity in the colon.Methods: Two groups of infants fed infant formula with 0.8 g/100 ml GOS/FOS in a ratio of 9:1 (OSF group), or control formula (SF group) were evaluated in a randomised, double blind, placebo controlled intervention study. A breast-fed group was studied in parallel. At study onset and after 4 and 6 weeks, faecal samples were examined for the number of bifidobacteria, pH, short chain fatty acids and lactate.Results: After 6 weeks, the mean proportion of bifidobacteria was significantly higher in the OSF group (59.6% versus 49.5% in the SF group; P < 0.05). Compared with controls, infants in the OSF group had a lower stool mean pH and an increased proportion of acetate and a decreased proportion of propionate. The mean pH in the OSF and SF groups were 5.7 and 6.3, respectively (P < 0.001).Conclusions: The addition of the prebiotic GOS/FOS mixture to an infant formula has a stimulating effect on the growth of bifidobacteria and on the metabolic activity of the total intestinal flora. The changes in short chain fatty acids, lactate and pH in the prebiotic group represent a fermentation profile that is closer to that observed in breast-fed infants compared to infants fed control formula.