Characterization of the insulin and insulin-like growth factor receptors and responsitivity of a fibroblast/adipocyte cell line before and after differentiation.
Characterization of the insulin and insulin-like growth factor receptors and responsitivity of a fibroblast/adipocyte cell line before and after differentiation.
复制标题
胰岛素和胰岛素样生长因子受体的表征以及成纤维细胞/脂肪细胞系分化前后的反应性。
DOI：
10.1016/0006-291x(86)91246-5
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Roth,RA
中科院分区：
文献类型：
--
作者：
Shimizu,M;Torti,F;Roth,RA
摘要 TA1细胞与3T3-L1细胞一样，在体外经历从成纤维细胞向脂肪细胞表型的分化过程。研究发现 TA1 前脂肪细胞的胰岛素受体数量较少，但胰岛素样生长因子 (IGF) I 和 II 的受体数量较多。此外，通过刺激葡萄糖或氨基酸摄取来测量，前脂肪细胞对 IGF 的反应比胰岛素更敏感。分化后，脂肪细胞具有更多数量的胰岛素受体，并且对胰岛素的响应性比 IGF-I 更好。这些结果表明胰岛素样生长因子是前脂肪细胞的主要调节剂，而胰岛素则调节脂肪细胞。
Abstract TA1 cells, like 3T3-L1 cells, undergo a differentiation process in vitro from a fibroblast to an adipocyte phenotype. The TA1 pre-adipocytes were found to have low numbers of insulin receptors but high numbers of receptors for insulin-like growth factors (IGF) I and II. Also, the pre-adipocytes were more responsive to IGF than insulin as measured by either stimulation of glucose or amino acid uptake. After differentiation, the adipocytes had higher numbers of insulin receptors and a better responsitivity to insulin than to IGF-I. These results indicate that insulin-like growth factors are the primary regulators of the pre-adipocytes whereas insulin regulates the adipocytes.