Newer laboratory methods for assessing nutriture of selected B-complex vitamins.
Newer laboratory methods for assessing nutriture of selected B-complex vitamins.
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DOI：
10.1146/annurev.nu.04.070184.002113
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发表时间：
1984
影响因子：
8.9
通讯作者：
H. Sauberlich
中科院分区：
文献类型：
--
作者：
H. Sauberlich
本综述仅限于新的或改进的实验室程序的发展进展，以评估硫胺素、核黄素、烟酸、维生素B6和叶酸的营养。其他维生素在这方面也取得了显著进展。在营养评估中应用的放射性测定技术和高效液相色谱方法的可用性增加是重要的。然而，对于一些维生素，仍然需要额外的方法来提供功能上可解释的临床前信息，并对营养物质的身体储备进行准确评估。通常用于解释所获得信息的指南是暂定的，需要确认或修订。由于经常缺乏质量控制和实验室间验证的合适参考标准，这种情况进一步复杂化。
This review is limited to progress in the development of new or improved laboratory procedures to assess the nutriture of thiamin, riboflavin, niacin, vitamin B6, and folic acid. There has been marked progress in this area for the other vitamins as well. The increased availability of radioassay techniques and HPLC methodologies that have application in nutrition assessment is significant. However, for a number of the vitamins, there is still a need for additional methods that provide functionally interpretable preclinical information and give accurate assessment of body reserves of the nutrient. Often the guides used to interpret the information obtained are tentative and require validation or revision. This situation is further complicated by the frequent lack of suitable reference standards for quality controls and interlaboratory validation.