The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3
The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3
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DOI：
10.1182/blood.v97.10.3303
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发表时间：
2001-05-15
期刊：
影响因子：
20.3
通讯作者：
Sano, K
中科院分区：
文献类型：
--
作者：
Bühring, HJ;Seiffert, M;Sano, K
最近有研究表明，单克隆抗体（MoAb） 97A6可检测在嗜碱性细胞及其CD34（+）前体细胞以及嗜碱性细胞系KU-812上表达的表面抗原。本文测定了亲和层析法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法从KU-812裂解物分离的97A6抗原的部分氨基酸序列。用高效液相色谱法对130-和150-kd波段的色氨酸进行序列比对，发现与外核苷酸焦磷酸酶/磷酸二酯酶-3 （E-NPP3;, CD203c）的393 ~ 405氨基酸100%一致，但与相关的外酶PC-1 （E-NPP1）的氨基酸不一致。此外，MoAb 97A6选择性识别转染了人E-NPP3的293细胞，但对转染了PC-1或亲本293细胞的细胞没有反应。此外，在嗜碱性细胞中检测到E-NPP3信使RNA的表达，而在其他外周血细胞中未检测到。最后，MoAb 97A6在KU-812细胞和外周血嗜碱性粒细胞中免疫沉淀磷酸二酯酶活性，而在其他细胞群中没有。这些数据表明，MoAb 97A6识别具有功能活性的II型跨膜酶E-NPP3。（Blood. 2001;97:3303-3305） (C) 2001年由美国血液学会出版。
It has recently been shown that monoclonal antibody (MoAb) 97A6 detects a surface antigen expressed on basophils and their CD34(+) precursor cells, as well as the basophil cell line KU-812. In this report the partial amino acid sequence of affinity chromatography- and sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated 97A6 antigen(s) from KU-812 lysates was determined. Sequence alignment of high-performance liquid chromatography-selected tryptic peptides from the resulting 130- and 150-kd bands revealed a 100% identity with amino acids 393 to 405 of ectonucleotide pyrophosphatase/phosphodiesterase-3 (E-NPP3;, CD203c) but not of the related ectoenzyme PC-1 (E-NPP1). Moreover, MoAb 97A6 selectively recognized 293 cells transfected with human E-NPP3, but did not react with cells transfected with PC-1 or parental 293 cells. In addition, E-NPP3 messenger RNA expression was detected in basophils but not other peripheral blood cells. Finally, MoAb 97A6 immunoprecipitated phosphodiesterase activity from KU-812 cells and peripheral blood basophils, but not from other cell populations. These data demonstrate that MoAb 97A6 recognizes the functionally active type II transmembrane ectoenzyme E-NPP3. (Blood. 2001;97:3303-3305) (C) 2001 by The American Society of Hematology.