PDZ-binding kinase participates in spermatogenesis
PDZ-binding kinase participates in spermatogenesis
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PDZ 结合激酶参与精子发生
DOI：
10.1016/s1357-2725(01)00005-x
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发表时间：
2001-06-01
影响因子：
4
通讯作者：
Mao, YM
中科院分区：
文献类型：
--
作者：
Zhao, SH;Dai, JL;Mao, YM
从人胎脑cDNA文库中分离到一个克隆，命名为SPK(精子发生相关蛋白激酶)。该蛋白与人PDZ结合蛋白(PBK)有99%的同源性，是一种有丝分裂的蛋白。在本研究中还分离到了其3‘端长约300个碱基的异构体。SPE：通过辐射杂交将该基因定位于人染色体8p21.2。Northern印迹分析表明，SPK基因主要在睾丸中表达。原位杂交结果显示SPK mRNA定位于曲细精管外层细胞层，提示其参与了生精过程。(C)2001爱思唯尔科学有限公司。保留所有权利。
A cDNA clone denoted SPK (spermatogenesis-related protein kinase) was isolated from human fetal brain cDNA library. The deduced protein was 99% identical to human PDZ-binding kinase (PBK), which is a mitotic kinase. Its isoform, which is about 300 bp longer at 3 ' end, was also isolated in this study. SPE: gene was assigned to human chromosome 8p21.2 by radiation hybrid. Northern blot analysis indicated that SPK mRNA was predominantly expressed in testis. SPK mRNA was localized to the outer cell layer of seminiferous tubules by in situ hybridization, suggesting involvement of it in the process of spermatogenesis. (C) 2001 Elsevier Science Ltd. All rights reserved.