Intestinal Dysbiosis and Yeast Isolation in Stool of Subjects with Autism Spectrum Disorders
Intestinal Dysbiosis and Yeast Isolation in Stool of Subjects with Autism Spectrum Disorders
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DOI：
10.1007/s11046-016-0068-6
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发表时间：
2017-04-01
期刊：
影响因子：
5.5
通讯作者：
Bravaccio, Carmela
中科院分区：
文献类型：
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作者：
Iovene, Maria Rosaria;Bombace, Francesca;Bravaccio, Carmela
通过简单的培养方法，ASD受试者中胃肠道酵母菌的存在频率较高（57.5%的ASD患者中有念珠菌，没有对照组）；光镜下假丝酵母菌呈侵袭型（假菌丝呈现），有利于与肠黏膜的粘附。由于乳酸杆菌和梭状芽胞杆菌数量的减少，维持了生态失调。在自闭症患者和对照组中也没有发现艰难梭菌及其毒素。轻度的肠道炎症和增强的肠通透性与胃肠道症状的存在一起被证实。疾病严重程度（CARs评分）与钙保护蛋白和梭状芽孢杆菌的存在存在显著的线性相关。此外，胃肠道症状，如便秘和肠交替，确实与乳果糖渗透性增加相关（多变量分析）。目前的数据为恢复asd患者肠道稳态的可能的特异性治疗干预提供了理论基础。
High frequency of gastrointestinal yeast presence in ASD subjects was shown through a simple cultural approach (Candida spp. in 57.5 % of ASDs and no controls); the identification of aggressive form (pseudo-hyphae presenting) of Candida spp. at light microscope means that adhesion to intestinal mucosa is facilitated. Dysbiosis appears sustained by lowered Lactobacillus spp. and decreased number of Clostridium spp. Absence of C. difficilis and its toxins in both ASDs and controls is also shown. Low-mild gut inflammation and augmented intestinal permeability were demonstrated together with the presence of GI symptoms. Significant linear correlation was found between disease severity (CARs score) and calprotectin and Clostridium spp. presence. Also GI symptoms, such as constipation and alternating bowel, did correlate (multivariate analyses) with the increased permeability to lactulose. The present data provide rationale basis to a possible specific therapeutic intervention in restoring gut homeostasis in ASDs.