Immunolocalization of vesicular glutamate transporter 2 and exocytosis‐related proteins in afferent nerve endings innervating taste buds in the rat incisive papilla
Immunolocalization of vesicular glutamate transporter 2 and exocytosis‐related proteins in afferent nerve endings innervating taste buds in the rat incisive papilla
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大鼠切牙乳头支配味蕾的传入神经末梢中囊泡谷氨酸转运蛋白 2 和胞吐作用相关蛋白的免疫定位
DOI：
10.1111/ahe.12911
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Saino Tomoyuki
中科院分区：
文献类型：
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作者：
Yokoyama Takuya;Ito Motoi;Yamamoto Yoshio;Hirakawa Masato;Sakanoue Wakana;Sato Kenichi;Saino Tomoyuki
本研究旨在研究囊泡谷氨酸转运蛋白（VGLUT）1和2以及与胞吐相关的蛋白质，即，可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体复合物（25 kDa突触体相关蛋白、突触融合蛋白1和囊泡相关膜蛋白2）和突触结合蛋白-1（Syt 1）的核心成分，在大鼠的切开乳头状味蕾中使用双间接免疫荧光法。未观察到VGLUT 1免疫反应性，而VGLUT 2免疫反应性点状产物与味蕾中的鸟嘌呤核苷酸结合蛋白G（t）亚基α3免疫反应性细胞密切相关。VGLUT 2在表达P2 X3嘌呤受体的传入神经末梢中免疫定位。25 kDa的突触体相关蛋白、突触融合蛋白1和囊泡相关膜蛋白2免疫定位于含有VGLUT 2免疫反应产物的神经末梢以及味蕾中的少数细胞中。VGLUT 2在一些对Syt 1免疫反应的胚内神经末梢中共免疫定位，Syt 1是一种涉及囊泡膜融合的钙传感器。本研究结果表明，传入神经末梢支配的切入味蕾释放谷氨酸的胞吐调节味觉细胞的功能。
The present study aimed to investigate the immunolocalization of vesicular glutamate transporter (VGLUT) 1 and 2, and proteins associated with exocytosis, i.e., core components of the solubleN‐ethylmaleimide‐sensitive factor attachment protein receptor complex (synaptosomal‐associated protein of 25 kDa, syntaxin 1, and vesicle‐associated membrane protein 2) and synaptotagmin‐1 (Syt1), in incisive papillary taste buds of rats using double‐indirect immunofluorescence. No VGLUT1 immunoreactivity was observed, whereas VGLUT2‐immunoreactive punctate products were closely associated with guanine nucleotide‐binding protein G(t) subunit α3‐immmunoreactive cells in taste buds. VGLUT2 was immunolocalized in P2X3 purinoceptor‐expressing afferent nerve endings. Synaptosomal‐associated protein of 25 kDa, syntaxin 1, and vesicle‐associated membrane protein 2 were immunolocalized in nerve endings containing VGLUT2‐immunoreactive products as well as a few cells in taste buds. VGLUT2 was co‐immunolocalized in some intragemmal nerve endings immunoreactive for Syt1, a calcium sensor implicated in vesicle membrane fusion. The present results suggest that afferent nerve endings innervating incisive taste buds release glutamate by exocytosis to modulate taste cell function.