Molecularly imprinted photoelectrochemical sensor for carcinoembryonic antigen based on polymerized ionic liquid hydrogel and hollow gold nanoballs/MoSe2 nanosheets.
Molecularly imprinted photoelectrochemical sensor for carcinoembryonic antigen based on polymerized ionic liquid hydrogel and hollow gold nanoballs/MoSe2 nanosheets.
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DOI：
10.1016/j.aca.2019.09.029
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发表时间：
2019-12
影响因子：
6.2
通讯作者：
Caiyun Wang;Yanying Wang;Huijuan Zhang;Hongping Deng;X. Xiong;Chunya Li;Wenwen Li
中科院分区：
文献类型：
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作者：
Caiyun Wang;Yanying Wang;Huijuan Zhang;Hongping Deng;X. Xiong;Chunya Li;Wenwen Li
以癌胚抗原（CEA）印迹聚合离子液体水凝胶为识别元件，空心金纳米球/MoSe 2纳米片为光敏元件，成功构建了光电化学（PEC）传感平台。为了在室温下完成印迹过程，保持癌胚抗原的生物活性和构象，提高其传感性能，合成了3-{[{4-N，N-双[（氨甲酰基）甲基丙烯酸乙酯]丁基}（（氨甲酰基）氨基）甲基丙烯酸乙酯]丙基}-1-乙烯基-1H-咪唑-3-溴化鎓（BCCPEimBr）离子液体。以BCCPEimBr离子液体为功能单体，CEA为模板，在中空金纳米球/MoSe 2纳米片修饰玻碳电极表面制备了分子印迹水凝胶膜。去除模板后，成功制备了CEA印迹光电化学传感器。在优化条件下，该传感器对CEA具有良好的选择性、灵敏度和稳定性，在0.05 ~ 5.0 ng mL− 1浓度范围内线性响应良好，检测限为11.2 pg mL−1（S/N = 3）。将该印迹PEC传感器用于临床人血清样品中CEA的准确测定。印迹PEC传感平台的过程可以用于其他生物分子，只需取代模板。
Using carcinoembryonic antigen (CEA) imprinted polymerized ionic liquid hydrogel as a recognition element and hollow gold nanoballs/MoSe2nanosheets as a photoactive element, a photoelectrochemical (PEC) sensing platform was successfully fabricated. To accomplish the imprinted process under room temperature to maintain the biological activity and the configuration of CEA, and thus to improve the sensing performances, 3-{[{4-N,N-Bis[(carbamoyl)ethylmethacrylate]butyl}((carbamoyl)amino)ethyl methacrylate] -propyl}-1-ethenyl-1H-imidazol-3-ium bromide (BCCPEimBr) ionic liquid was synthesized. By using BCCPEimBr ionic liquid as the functional monomer, CEA as the template, a molecularly imprinted hydrogel film was prepared on a hollow gold nanoballs/MoSe2nanosheets modified glassy carbon electrode surface. After removing the template, a CEA imprinted photoelectrochemical sensor was successfully fabricated. The imprinted PEC sensor shows good selectivity, sensitivity and stability towards CEA, produces a linear response in the concentration range from 0.05 to 5.0 ng mL−1and shows a detection limit of 11.2 pg mL−1(S/N = 3) under the optimized conditions. The imprinted PEC sensor was used to determine CEA in clinical human serum samples accurately. The procedure for the imprinted PEC sensing platform can be used for other biomolecules just by substituting the template.