Intestinal absorption of free oleic acid in the unanesthetized rat: evidence for a saturable component?
Intestinal absorption of free oleic acid in the unanesthetized rat: evidence for a saturable component?
复制标题
未麻醉大鼠肠道吸收游离油酸：可饱和成分的证据？
DOI：
10.1139/y84-190
复制
发表时间：
1984
影响因子：
2.1
通讯作者：
Dadufalza,VD
中科院分区：
文献类型：
--
作者：
Hollander,D;Chow,SL;Dadufalza,VD
本文研究了低浓度游离油酸在非麻醉大鼠肠腔中的吸收及管腔因子对其吸收的影响。油酸浓度（30-2500 μM）与其萌发率的关系符合二次曲线（y=x/（2.19 + 0.0015x），r= 0.94）。 油酸的吸收速率随着氢离子和牛磺胆酸钠浓度的增加而增加，或者随着未搅拌水层的厚度和电阻的减小而增加，或者在加入溶血素之后增加。添加人工洗涤剂吐温-80，或卵磷脂和亚油酸，亚麻酸和花生四烯酸的灌注降低油酸的吸收率。得出的结论是，在这个浓度范围内的油酸吸收显示出明显的饱和动力学，这是由于未搅拌层的影响，有限的水溶性油酸和可能饱和的细胞质脂肪酸结合蛋白。增加油酸的质子化浓度或减小未搅拌层阻力的因素提高其吸收速率，而提高其胶束溶解度或干扰其转移出细胞膜的因素降低其总吸收速率。
The intestinal absorption of free oleic acid at low intraluminal concentrations and the influence of luminal factors on its absorption were studied in the unanesthetized rat. The relationship between oleic acid concentration (30–2500 μM) and its rate of absorptions fitted best to a rectangular hyperbola (y=x/(2.19 + 0.0015x),r= 0.94). Oleic acid's rate of absorption increased as the hydrogen ion and sodium taurocholate concentrations were increased or as the thickness and resistance of the unstirred water layer were diminished or following the addition of lysolecithin. The additions of the artificial detergent Tween-80, or lecithin and linoleic, linolenic and arachidonic acids to the perfusate decreased oleic acid's rate of absorption. It was concluded that oleic acid absorption in this range of concentrations displays apparent saturation kinetics which are due to unstirred layer effects, limited aqueous solubility of oleic acid and possible saturation of cytosol fatty acid binding proteins. Factors which increase oleic acid's protonated concentration or diminish the unstirred layer resistance, enhance its absorption rate, while factors which enhance its micellar solubility or interfere with its transfer out of the cell membrane decrease its overall rate of absorption.