ISOLATION AND CHARACTERIZATION OF A ZINC FINGER POLYPEPTIDE GENE AT THE HUMAN CHROMOSOME-11 WILMS TUMOR LOCUS
ISOLATION AND CHARACTERIZATION OF A ZINC FINGER POLYPEPTIDE GENE AT THE HUMAN CHROMOSOME-11 WILMS TUMOR LOCUS
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DOI：
10.1016/0092-8674(90)90601-a
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发表时间：
1990-02-09
期刊：
影响因子：
64.5
通讯作者：
HOUSMAN, DE
中科院分区：
文献类型：
--
作者：
CALL, KM;GLASER, T;HOUSMAN, DE
我们已经分离了一系列基因组和cDNA克隆，这些克隆定位在构成和肿瘤缺失的边界内，定义了人类染色体11(带p13)上的Wilms肿瘤位点。与这些克隆对应的转录单位跨度约50kb，编码约3kb长的mRNA。这种mRNA在有限的细胞类型中表达，主要在肾脏和一小部分造血细胞中表达。该基因编码的多肽具有许多特征，提示其在转录调控中具有潜在的作用。这包括存在四个锌指结构域和一个富含脯氨酸和谷氨酰胺的区域。预测的多肽的氨基酸序列与两个生长调节的哺乳动物多肽Egr1和EGR2具有显著的同源性。该基因的遗传定位，其组织特异性表达，以及从其序列预测的功能使我们认为它代表了11p13 Wilms‘肿瘤基因。
We have isolated a series of genomic and cDNA clones mapping within the boundaries of constitutional and tumor deletions that define the Wilms'' tumor locus on human chromsome 11 (band p13). The transcription unit corresponding to these clones spans approximately 50 kb and encodes an mRNA approximately 3 kb long. This mRNA is expressed in a limited range of cell types, predominantly in the kidney and a subset of hematopoietic cells. The polypeptide encoded by this locus has a number of features suggesting a potential role in transcriptional regulation. These include the presence of four zinc finger domains and a region rich in proline and glutamine. The amino acid sequence of the predicted polypeptide shows significant homology to two growth regulated mammalian polypeptides, EGR1 and EGR2. The genetic localization of this gene, its tissue-specific expression, and the function predicted from its sequence lead us to suggest that it represents the 11p13 Wilms'' tumor gene.