Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy.
Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy.
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通过原子力显微镜比较未成熟和成熟的骨髓源性树突状细胞。
DOI：
10.1186/1556-276x-6-455
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发表时间：
2011-07-16
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Xing F;Wang J;Hu M;Yu Y;Chen G;Liu J
首次通过原子力显微镜(AFM)对未成熟和成熟的骨髓来源的树突状细胞(BMDCs)进行了比较研究，以阐明它们的纳米结构和粘附力的差异。AFM图像显示，粒细胞-巨噬细胞集落刺激因子+IL-4处理的未成熟BMDCs主要呈圆形，表面光滑，而经内毒素诱导的成熟BMDCs除变大、变平、变长外，还呈不规则形状，细胞膜上有大量伪足或板足及褶皱。AFM定量分析进一步表明，成熟BMDCs的表面粗糙度大大增加，其粘附力是未成熟BMDCs的4倍。成熟BMDCs产生的高水平IL-12和表面MHC-II的高表达支持了成熟BMDCs的纳米特征。这些发现为了解未成熟和成熟的骨髓基质细胞的纳米结构提供了新的视角。
A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs.