Effects of betaine on ethanol-stimulated secretion of IGF-I and IGFBP-1 in rat primary hepatocytes: involvement of p42/44 MAPK activation
Effects of betaine on ethanol-stimulated secretion of IGF-I and IGFBP-1 in rat primary hepatocytes: involvement of p42/44 MAPK activation
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DOI：
10.3748/wjg.v12.i11.1718
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发表时间：
2006-03-21
影响因子：
4.3
通讯作者：
Kang, Chang-Won
中科院分区：
文献类型：
--
作者：
Lee, Myeong Soo;Kim, Myung-Sunny;Kang, Chang-Won
目的：采用放射免疫法和Western blotting法分别观察甜菜碱对乙醇诱导的原代培养大鼠肝细胞分泌IGF-I和IGFBP-1的影响。用不同浓度的甜菜碱（10、10（-4）和10（-3）mol/L）处理肝细胞。结果：乙醇对原代培养大鼠肝细胞IGF-I分泌的抑制作用可被甜菜碱以浓度依赖性方式减弱。10 - 3mol/L甜菜碱显著增加IGF-I的分泌，但降低IGFBP-1的分泌。10（-3）mol/L甜菜碱处理后10 min至5 h，p42/44丝裂原活化蛋白激酶（MAPK）活性显著增强。此外，在IGF-1和IGFBP-1分泌的变化所造成的增加甜菜碱诱导的p42/44 MAPK活性在原代培养的大鼠肝细胞被阻断治疗与MAPK抑制剂PD98059。结论：甜菜碱通过激活p42/44 MAPK调节原代培养大鼠肝细胞IGF-I和IGFBP-1的分泌。甜菜碱还改变乙醇诱导的MAPK激活。(c)2006年，WJG出版社。All rights reserved.
AIM: To evaluate the effects of betaine on the ethanol-induced secretion of IGF-I and IGFBP-1 using radioimmunoassay and Western blotting, respectively, in primary cultured rat hepatocytes.METHODS: Hepatocytes isolated from male Sprague-Dawley rats were incubated with various concentrations of ethanol and PD98059 procedures. The hepatocytes were also treated with different doses of betaine (10, 10(-4), and 10(-3) mol/L). We measured IGF-I and IGFBP-1 using radioimmunoassay and Western blotting, respectively.RESULTS: The ethanol-induced inhibition of IGF-I secretion was attenuated by betaine in a concentration-dependent manner in primary cultured rat hepatocytes. At 10-3 mol/L, betaine significantly increased IGF-I secretion but decreased IGFBP-1 secretion. In addition, p42/44 mitogen-activated protein kinase (MAPK) activity was accelerated significantly from 10 min to 5 h after treatment with 10(-3) mol/L betaine. Furthermore, the changes in IGF-1 and IGFBP-1 secretion resulting from the increased betaine-induced p42/44 MAPK activity in primary cultured rat hepatocytes was blocked by treatment with the MAPK inhibitor PD98059. Betaine treatment blocked the ethanol-induced inhibition of IGF-I secretion and p42/44 MAPK activity, and the ethanol-induced increase in IGFBP-1 secretion.CONCLUSION: Betaine modulates the secretion of IGF-I and IGFBP-1 via the activation of p42/44 MAPK in primary cultured rat hepatocytes. Betaine also alters the MAPK activations induced by ethanol. (c) 2006 The WJG Press. All rights reserved.