Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian
Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian
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DOI：
10.1016/j.gep.2010.12.004
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发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Okamura, Yasushi
中科院分区：
文献类型：
--
作者：
Ogasawara, Michio;Sasaki, Mari;Okamura, Yasushi
VSP是一种跨膜蛋白，其胞质区域显示出与10号染色体上缺失的磷酸酶和张力蛋白同源物（PTEN）的显著相似性。值得注意的是，VSP表现出独特的能力，通过耦合跨膜电压传感器结构域的PTEN样磷酸肌醇磷酸酶结构域的电信号转化为磷酸肌醇营业额。此外，VSP基因是已知的广泛保守的后口类基因组中，虽然VSP在体内的功能仍然在很大程度上未知。本文研究了海鞘VSP在海洋无脊椎脊索动物玻璃海鞘胚胎和幼体中的表达模式。RT-PCR结果表明，Ci-VSP在幼虫期表达，并持续表达在青少年。整体原位杂交显示，Ci-VSP在2至3周龄青少年的胃、肠细胞和血细胞中表达。此外，用Ci-VSP和Ci-PTEN探针对来自2个月大的成人的血细胞进行双重染色显示，Ci-VSP阳性细胞是与表达Ci-PTEN的细胞分开的不同群体。这些发现表明，除了先前提出的在睾丸或精子中的作用之外。Ci-VSP在消化系统和血液细胞中的电压诱导的信号转导中起关键作用。(C)2011 Elsevier B. V.保留所有权利。
VSP is a transmembrane protein whose cytoplasmic region shows significant similarity to phosphatase and tensin homolog deleted on chromosome 10 (PTEN). Notably, VSP exhibits a unique ability to transduce electrical signals into phosphoinositide turnover by coupling a transmembrane voltage sensor domain to the PTEN-like phosphoinositide phosphatase domain. Moreover, VSP gene is known to be widely conserved among deuterostome genomes, though the function of VSP in vivo remains largely unknown. In the present study, the expression pattern of ascidian VSP(Ci-VSP) was examined in embryos and juveniles of a marine invertebrate chordate, Ciona intestinalis. RT-PCR showed that Ci-VSP is expressed at the larval stage and that expression persists in juveniles. Whole mount in situ hybridization showed that Ci-VSP is expressed in cells of the stomach, intestine and blood cells of 2- to 3-week-old juveniles. Moreover, double staining blood cells from 2-month-old adults with Ci-VSP and Ci-PTEN probes showed that Ci-VSP-positive cells are a distinct population, separate from cells expressing Ci-PTEN. These findings suggest that in addition to its previously suggested roles in testis or sperm. Ci-VSP plays a key role in voltage-induced signal transduction in cells of the digestive system and blood. (C) 2011 Elsevier B.V. All rights reserved.