THE EFFECTS OF PLATELET-DERIVED GROWTH-FACTOR IN CULTURED MICROVESSEL ENDOTHELIAL-CELLS
THE EFFECTS OF PLATELET-DERIVED GROWTH-FACTOR IN CULTURED MICROVESSEL ENDOTHELIAL-CELLS
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DOI：
10.1210/endo-124-4-1841
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发表时间：
1989-04-01
期刊：
影响因子：
4.8
通讯作者：
HART, MN
中科院分区：
文献类型：
--
作者：
BAR, RS;BOES, M;HART, MN
本文研究了血小板源性生长因子（PDGF）对大血管（牛主动脉和肺动脉）和微血管（牛脂肪和小鼠脑）内皮细胞DNA胸苷掺入和葡萄糖及中性氨基酸摄取的影响。与以前的研究类似。PDGF不与大血管细胞结合，不影响其代谢功能。相反，PDGF特异性地结合到两种类型的微血管细胞培养物中，并且在这些细胞中还刺激葡萄糖和中性氨基酸的摄取以及胸苷掺入DNA中。PDGF的刺激作用发生在2 ng/ml的浓度下，对于氨基异丁酸和葡萄糖摄取，最大刺激高达对照值的5倍，对于胸苷掺入，最大刺激高达8至10倍。PDGF的最大作用是添加到胰岛素样生长因子I的那些，胰岛素样生长因子I是微血管内皮细胞中所有三个代谢过程的已知刺激物。在示踪剂结合的量和结合的亲和力方面，PDGF与内皮细胞的结合通常等同于PDGF与人包皮成纤维细胞的结合。重组和血小板衍生的PDGF也有类似的作用。我们的结论是，这两个培养的微血管内皮细胞不仅产生PDGF样物质，但能够结合和响应PDGF。
The effects of platelet-derived growth factor (PDGF) on thymidine incorporation into DNA and glucose and neutral amino acid uptake were studied in endothelial cells cultured from macrovessels (bovine aorta and pulmonary artery) and microvessels (bovine fat and mouse brain). Similar to previous studies. PDGF did not bind to macrovessel cells, not did it influence their metabolic function. In contrast, PDGF bound specifically to the two types of microvessel cell culture and in these cells also stimulated the uptake of glucose and neutral amino acids as well as the incorporation of thymidine into DNA. Stimulatory effects of PDGF occurred at concentrations of 2 ng/ml, with maximal stimulation up to 5-fold of the control value for amino isobutyric acid and glucose uptake and up to 8- to 10-fold for thymidine incorporation. The maximal effects of PDGF were additive to those of insulin-like growth factor I, a known stimulator of all three metabolic processes in microvessel endothelial cells. the binding of PDGF to the endothelial cells was, in general, equivalent to PDGF binding to human foreskin fibroblasts, both in the magnitude of tracer binding and in the affinity of binding. Similar effects were found with recombinant and platelet-derived PDGF. We conclude that these two cultured microvessel endothelial cell not only produce PDGF-like material, but are capable of binding and responding to PDGF.