Isolation of Marine Birnavirus from Sea Squirts Halocynthia roretzi
Isolation of Marine Birnavirus from Sea Squirts Halocynthia roretzi
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从海鞘 Halocynthia roretzi 中分离海洋双病毒
DOI：
10.1007/978-4-431-66982-1_64
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
S. Suzuki
中科院分区：
文献类型：
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作者：
Sung‐Ju Jung;M. Oh;T. Date;S. Suzuki
自1995年以来，在韩国南部沿海地区发生了罗氏盐辛西娅（Halocynthia roretzi）的大量死亡。我们从病H中分离到一株病毒（UR 1）。roretziin 1999年5月，并进行了病毒特性，血清学和分子生物学调查。从大鳞鲑胚胎（CHSE-214）和虹鳟性腺（RTG-2）细胞系的消化腺和咽缝中分离到UR 1。病毒颗粒无包膜，外观呈六边形，平均直径为63 nm。UR 1的RNA由两段组成，为双RNA病毒科的特征。UR 1与MABV株血清学相似，而与IPNV株血清学相似。采用逆转录-聚合酶链反应（RT-PCR）技术，对A基因组上470 bp的VP 5区、770 bp的VP 2区和643 bp的VP 3区进行了序列测定。与MABV Y-6和NCI的同源性均在97.5%以上。结果表明，该菌株为aH.罗雷兹是MABV的成员。这是首次从海鞘中分离到病毒。
Mass mortality of the culturedHalocynthia roretzihas been occurred in south coastal regions of Korea since 1995. We isolated a virus (UR1) from diseasedH. roretziin May of 1999 and conducted virus characterization, serological and molecular biological investigations. UR1 was isolated from digestive gland and pharyngeal slit in chinook salmon embryo (CHSE-214) and rainbow trout gonad (RTG-2) cell line. The virus particles were unenveloped, hexagonal in appearance and had an average diameter of 63 nm. The RNA of UR1 consisted of 2 segments as a characteristic of birnaviridae. The UR1 was serologically similar to MABV strains other than IPNV. Reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the 470bp of VP5, 770bp of internal VP2 region, and 643bp of VP3 region on genome segment A were carried out. The homology of UR1 with MABV Y-6 and NCI was over 97.5 % in all the regions mentioned above. It was concluded that the isolate from aH. roretziis a member of MABV. It is the first report of a virus isolation from ascidians.