Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan
Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan
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DOI：
10.1186/1756-3305-3-10
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发表时间：
2010-02-25
影响因子：
3.2
通讯作者：
Hashiguchi, Yoshihisa
中科院分区：
文献类型：
--
作者：
Kato, Hirotomo;Uezato, Hiroshi;Hashiguchi, Yoshihisa
在巴基斯坦皮肤利什曼病流行的沙漠地区，调查了利什曼原虫自然感染血吸虫沙蝇的情况。在解剖的220只雌性沙蝇中，有一只沙蝇的后肠鞭毛呈阳性。经细胞色素b(Cytb)、糖体甘油醛磷酸脱氢酶(GGAPDH)和小亚基核糖体RNA(SSU RRNA)基因序列分析，该寄生虫可能是爬行动物或两栖动物的锥虫种。这是巴基斯坦首次报道血吸虫沙蝇自然感染一种锥虫物种。在利什曼病流行地区进行媒介物种流行病学研究时，应考虑到沙蝇感染锥虫物种的可能性。
The natural infection of phlebotomine sand flies by Leishmania parasites was surveyed in a desert area of Pakistan where cutaneous leishmaniasis is endemic. Out of 220 female sand flies dissected, one sand fly, Phlebotomus kazeruni, was positive for flagellates in the hindgut. Analyses of cytochrome b (cyt b), glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and small subunit ribosomal RNA (SSU rRNA) gene sequences identified the parasite as a Trypanosoma species of probably a reptile or amphibian. This is the first report of phlebotomine sand flies naturally infected with a Trypanosoma species in Pakistan. The possible infection of sand flies with Trypanosoma species should be taken into consideration in epidemiological studies of vector species in areas where leishmaniasis is endemic.