An adenosine deaminase that generates inosine at the wobble position of tRNAs
An adenosine deaminase that generates inosine at the wobble position of tRNAs
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DOI：
10.1126/science.286.5442.1146
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发表时间：
1999-11-05
期刊：
影响因子：
56.9
通讯作者：
Keller, W
中科院分区：
文献类型：
--
作者：
Gerber, AP;Keller, W
几种转运RNA（tRNA）首先含有肌苷（I）。其反密码子的位置（位置34）：认为该修饰。以扩大蛋白质合成过程中的密码子识别能力。描述了在tRNA中形成I-34的酿酒酵母的tRNA特异性腺苷脱氨酶。异源二聚体酶由两个序列相关的亚基（Tad 2 p/ADAT 2和Tad 3 p/ADAT 3）组成，这两个亚基都含有胞苷脱氨酶（CDA）基序。每个亚基由一个必需基因（TAD 2和TAD 3）编码，表明I-34是延伸tRNA中不可或缺的碱基修饰。这些结果提供了CDA超家族和RNA依赖性腺苷脱氨酶（ADAR/ADAT）之间的进化联系。
Several transfer RNAs (tRNAs) contain inosine (I) at the first. position of their anticodon (position 34): this modification is thought. to enlarge the codon recognition capacity during protein synthesis. The tRNA-specific adenosine deaminase of Saccharomyces cerevisiae that forms I-34 in tRNAs is described. The heteroclimeric enzyme consists of two sequence-related subunits (Tad2p/ADAT2 and Tad3p/ADAT3), both of which contain cytidine deaminase (CDA) motifs. Each subunit is encoded by an essential gene (TAD2 and TAD3), indicating that I-34 is an indispensable base modification in elongating tRNAs. These results provide an evolutionary Link between the CDA superfamily and RNA-dependent adenosine deaminases (ADARs/ADATs).