Iodination of human thyroglobulin (Tg) alters its immunoreactivity. II. Fine specificity of a monoclonal antibody that recognizes iodinated Tg.
Iodination of human thyroglobulin (Tg) alters its immunoreactivity. II. Fine specificity of a monoclonal antibody that recognizes iodinated Tg.
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人甲状腺球蛋白 (Tg) 的碘化会改变其免疫反应性。
DOI：
10.1046/j.1365-2249.1998.00644.x
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发表时间：
1998
影响因子：
4.6
通讯作者：
Burek,CL
中科院分区：
文献类型：
--
作者：
Saboori,AM;Rose,NR;Burek,CL
在以前的研究中，我们发现，鼠单抗42 C3，提出了对人类Tg，不同的Tg识别取决于其碘化水平的Tg。这一发现的一个可能解释是碘直接参与了MoAb 42 C3识别的特异性表位。在本研究中，我们报告了单克隆抗体42 C3与碘化Tg的结合被T4、T3、反向T3（rT 3）、三碘甲腺乙酸（triac）、二碘甲状腺原氨酸（T2）、二碘酪氨酸（DIT）抑制，但不被甲状腺原氨酸（T0）或酪氨酸抑制。这些碘化化合物的抑制顺序为T_4> T_3> rT_3> triac> T_2> DIT。 MoAb 42 C3不具有与T3、T4-受体相同的特异性，因为这些碘化化合物在受体上的结合顺序与它们对该MoAb的抑制顺序不同。单克隆抗体42 C3也识别随后在体外碘化的非碘化Tg。它不能识别另一种蛋白质，牛血清白蛋白，用同样的方法在体外碘化。这些结果表明碘化酪氨酸和甲状腺原氨酸决定了MoAb 42 C3的结合特异性。这些化合物对MoAb 42 C3的抑制作用取决于它们的碘含量以及碘在芳环中的位置。
In a previous investigation, we found that murine MoAb 42C3, raised against human Tg, recognized Tg differently depending upon its level of iodination of Tg. A possible explanation for this finding is that iodine is directly involved with the specific epitope recognized by MoAb 42C3. In the present study, we report that the binding of MoAb 42C3 to iodinated Tg is inhibited by T4, T3, reverse T3 (rT3), triiodothyroacetic acid (triac), diiodothyronine (T2), diiodotyrosine (DIT), but not by thyronine (T0) or tyrosine. The order of inhibition of these iodinated compounds is T4 > T3 > rT3 > triac > T2 > DIT. The MoAb 42C3 does not have the same specificity as the T3, T4-receptor since the order of binding of these iodinated compounds on the receptor differed from the order of their inhibition of this MoAb. Monoclonal antibody 42C3 also recognized non-iodinated Tg that was subsequently iodinatedin vitro. It failed to recognize another protein, bovine serum albumin, that was iodinatedin vitroby the same method. These results suggest that iodinated tyrosines and thyronines determine the binding specificity of MoAb 42C3. The inhibitory effects of these compounds on MoAb 42C3 depend on their iodine content as well as location of iodine in the aromatic ring.