The Akt and MAPK signal-transduction pathways regulate growth factor actions in isolated gastric parietal cells.
The Akt and MAPK signal-transduction pathways regulate growth factor actions in isolated gastric parietal cells.
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Akt 和 MAPK 信号转导途径调节离体胃壁细胞中生长因子的作用。
DOI：
10.1053/j.gastro.2004.06.059
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发表时间：
2004
期刊：
影响因子：
29.4
通讯作者：
Todisco,Andrea
中科院分区：
文献类型：
--
作者：
Stepan,Vinzenz;Pausawasdi,Nonthalee;Ramamoorthy,Saravanan;Todisco,Andrea
背景与目的将纯化的（>95%）犬壁细胞与表皮生长因子一起孵育7-16小时，刺激H+K+-腺苷三磷酸酶基因的表达。在这项研究中，我们研究了延长刺激（72小时）的壁细胞与表皮生长因子的效果。MethodsH+K+-腺苷三磷酸酶蛋白和基因表达进行了评估，免疫组化和北方印迹。促分裂原活化蛋白激酶和Akt活化通过激酶测定和特异性抗磷酸化抗体的Western印迹进行定量。Akt过表达实现了由腺病毒介导的基因转移的一个组成型活性Akt基因。结果表皮生长因子改变了培养细胞的形态，获得梭形细胞的外观，并抑制H+K+-腺苷三磷酸酶基因的表达。用抗H +K+-腺苷三磷酸酶抗体和德克萨斯红标记的双花扁豆凝集素对细胞进行染色，证实梭形细胞表达壁细胞分化的标志物。表皮生长因子刺激有丝分裂原活化蛋白激酶，在5分钟和72小时后观察到2个活化峰，而它在孵育5分钟后激活Akt，但在孵育72小时后不激活Akt。Akt的过表达阻断表皮生长因子诱导的形态转化和H+K+-腺苷三磷酸酶基因表达的抑制。在有丝分裂原活化蛋白激酶抑制剂PD 98059的存在下观察到相同的结果。结论Akt信号转导通路的激活可能是诱导壁细胞成熟和分化的关键事件。
Background & AimsIncubation of purified (>95%) canine parietal cells in primary culture with epidermal growth factor for 7–16 hours stimulates H+K+-adenosine triphosphatase gene expression. In this study, we examined the effect of prolonged stimulation (72 hours) of the parietal cells with epidermal growth factor.MethodsH+K+-adenosine triphosphatase protein and gene expression were assessed by immunohistochemistry and Northern blots. Mitogen-activated protein kinase and Akt activation were quantitated by kinase assays and Western blots with specific antiphospho antibodies. Akt overexpression was achieved by adenovirus-mediated gene transfer of a constitutively active Akt gene.ResultsEpidermal growth factor changed the morphology of the cultured cells, which acquired the appearance of fusiform cells, and it inhibited H+K+-adenosine triphosphatase gene expression. Staining of the cells both with anti–H+K+-adenosine triphosphatase antibodies and with Texas Red–labeled Dolichos biflorus lectin confirmed that the fusiform cells expressed markers of parietal cell differentiation. Epidermal growth factor stimulated mitogen-activated protein kinase with 2 peaks of activation, observed after 5 minutes and 72 hours, whereas it activated Akt after 5 minutes but not 72 hours of incubation. Overexpression of Akt blocked both epidermal growth factor–induced morphological transformation and inhibition of H+K+-adenosine triphosphatase gene expression. Identical results were observed in the presence of the mitogen-activated protein kinase inhibitor PD98059.ConclusionsActivation of the Akt signal-transduction pathway seems to be a crucial event for the induction of parietal cell maturation and differentiation.
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影响因子：
29.4
作者：
Wang, TC;Dangler, CA;Fox, JG
通讯作者：
Fox, JG
生长因子的非有丝分裂作用：其在肠道生理学和病理生理学中作用的综合观点。
DOI：
--
发表时间：
1997
期刊：
Gastroenterology.
影响因子：
--
作者：
Uribe,JM;Barrett,KE
通讯作者：
Barrett,KE
Carbachol 通过多种信号通路激活分离的胃壁细胞中的 ERK2。
DOI：
10.1152/ajpgi.1999.276.6.g1484
发表时间：
1999
期刊：
The American journal of physiology.
影响因子：
--
作者：
Takeuchi,Y;Pausawasdi,N;Todisco,A
通讯作者：
Todisco,A
DOI：
10.1007/978-88-470-1405-3_42
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Arieh Riskin;Carlo Agostoni;R. Shamir
通讯作者：
Arieh Riskin;Carlo Agostoni;R. Shamir
DOI：
10.1073/pnas.95.3.1091
发表时间：
1998-02-03
影响因子：
11.1
作者：
Cheng, MG;Sexl, V;Roussel, MF
通讯作者：
Roussel, MF