Functional studies on a ketoreductase gene from Streptomyces sp AM-7161 to control the stereochemistry in medermycin biosynthesis
Functional studies on a ketoreductase gene from Streptomyces sp AM-7161 to control the stereochemistry in medermycin biosynthesis
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DOI：
10.1016/j.bmc.2005.07.060
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发表时间：
2005-12-15
影响因子：
3.5
通讯作者：
Ichinose, K
中科院分区：
文献类型：
--
作者：
Li, AY;Itoh, T;Ichinose, K
麦德霉素在吡喃环中显示出与放线菌紫相同的反式（3S，15R）构型，这对其生物活性至关重要。一种麦德霉素生物合成基因 ined-ORF12 被认为参与 C-3 的立体化学控制。功能互补表明它与之前证明的 actVI-ORFI 发挥相似的作用，以确定放线菌紫素生物合成中 C-3 的立体特异性。 med-ORF12与放线菌紫素早期生物合成基因的共表达进一步证明med-ORF12编码酮还原酶，负责吡喃环形成中C-3处的对映选择性还原。 (c) 2005 Elsevier Ltd. 保留所有权利。
Medermycin shows the same trans (3S,15R) configuration as actinorhodin in the pyran ring crucial for its bioactivity. One medermycin biosynthetic gene, ined-ORF12, is assumed to be involved in the stereochemical control at C-3. Functional complementation suggested that it plays a similar role as actVI-ORFI previously proved to determine the stereospecificity at C-3 in actinorhodin biosynthesis. Co-expression of med-ORF12 with actinorhodin early biosynthetic genes further demonstrated that med-ORF12 encodes a ketoreductase responsible for the enantioselective reduction at C-3 in the formation of the pyran ring. (c) 2005 Elsevier Ltd. All rights reserved.